Pou?ití SSR markerů pro identifikaci odrůd révy registrovaných v České republice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F06%3A00094517" target="_blank" >RIV/62156489:43510/06:00094517 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Use of SSR markers to identify grapevine cultivars registered in the Czech republic

Popis výsledku v původním jazyce

In this study we analyzed 51 grapevine cultivars registered in the Czech Republic using six highly informative SSR loci, and determined SSR profiles typical for each cultivar. All cultivars were easily distinguished on the basis of SSR allele length, except for berry color mutants (Pinot and Chasselas group). The average number of detected alleles per locus was 13.2, while observed heterozygosity is between 0.745 (VrZAG 79) and 0.922 (VVMD 7). The lengths of the alleles were compared with results from other laboratories; lengths were either the same or different by 1-3 bp. We also tested a new approach to microsatellite data comparison using a coding system based on reference alleles as a size standard (THIS et al., 2004). The obtained profiles were mostly in accordance with profiles of reference cultivars (THIS et al., 2004); we discuss the possible reasons for observed discrepancies. A third allele in the microsatellite spectrum was observed in five samples where template DNA origina

Název v anglickém jazyce

Use of SSR markers to identify grapevine cultivars registered in the Czech republic

Popis výsledku anglicky

In this study we analyzed 51 grapevine cultivars registered in the Czech Republic using six highly informative SSR loci, and determined SSR profiles typical for each cultivar. All cultivars were easily distinguished on the basis of SSR allele length, except for berry color mutants (Pinot and Chasselas group). The average number of detected alleles per locus was 13.2, while observed heterozygosity is between 0.745 (VrZAG 79) and 0.922 (VVMD 7). The lengths of the alleles were compared with results from other laboratories; lengths were either the same or different by 1-3 bp. We also tested a new approach to microsatellite data comparison using a coding system based on reference alleles as a size standard (THIS et al., 2004). The obtained profiles were mostly in accordance with profiles of reference cultivars (THIS et al., 2004); we discuss the possible reasons for observed discrepancies. A third allele in the microsatellite spectrum was observed in five samples where template DNA origina

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/QC1156" target="_blank" >QC1156: Vytvoření alternativní metodiky identifikace odrůd révy vinné založené na zhodnocení rozdílností ve struktuře DNA u jednotlivých odrůd.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International journal of vine and wine sciences

ISSN

1151-0285

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

2

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

10

Strana od-do

2

Kód UT WoS článku

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EID výsledku v databázi Scopus

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