Stanovení Fusarium spp. a forma specialis pomocí RAPD a PCR markerů.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F06%3A00103982" target="_blank" >RIV/62156489:43510/06:00103982 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of Fusarium spp. and formae speciales using RAPD and PCR markers

Popis výsledku v původním jazyce

For the selection of resistent genotypes of breeding material in Callistephus chinensis NEES. against Fusarium strains, it was searched for specific active strains of this. Gene pool of Fusarium strains was collected, purified and tested with plant material. To declare differences between pathogenic and non-pathogenic isolates were genomic methods used. The RAPD and PCR technique (Moravcová et al. 2004, Pasquali et al. 2003) were used to analyse of genomic DNA of 40 fusarium strains, collected from different host plants, mainly Callistephus chinensis NEES. in comparison with 13 Fusarium species included F. oxysporum and its f.sp. callistephi, cyclaminis, dianthi, lycopersici, radicis-lycopersici, narcisi, pisi and tracheiphilum. These isolates are deposited in University fungal collection. 10 deca-hexamers (Pasquali et al. 2003, Hennig 1999) random primers and 2 pairs of specific primers were used for amplification in these study. New PCR markers based on conservative sequences in mito

Název v anglickém jazyce

Characterization of Fusarium spp. and formae speciales using RAPD and PCR markers

Popis výsledku anglicky

For the selection of resistent genotypes of breeding material in Callistephus chinensis NEES. against Fusarium strains, it was searched for specific active strains of this. Gene pool of Fusarium strains was collected, purified and tested with plant material. To declare differences between pathogenic and non-pathogenic isolates were genomic methods used. The RAPD and PCR technique (Moravcová et al. 2004, Pasquali et al. 2003) were used to analyse of genomic DNA of 40 fusarium strains, collected from different host plants, mainly Callistephus chinensis NEES. in comparison with 13 Fusarium species included F. oxysporum and its f.sp. callistephi, cyclaminis, dianthi, lycopersici, radicis-lycopersici, narcisi, pisi and tracheiphilum. These isolates are deposited in University fungal collection. 10 deca-hexamers (Pasquali et al. 2003, Hennig 1999) random primers and 2 pairs of specific primers were used for amplification in these study. New PCR markers based on conservative sequences in mito

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů