Comparison of Methods of Isolating DNA for ESFY Phytoplasma Detection

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of Methods of Isolating DNA for ESFY Phytoplasma Detection

Popis výsledku v původním jazyce

'Candidatus phytoplasma prunorum', (European stone fruit yellows phytoplasma - ESFY), is nowadays one of the most important diseases belonging to the list of organisms for which quarantine is required, and is a long-standing problem in apricot and peachorchards in the Czech Republic. Its detection and diagnosis is based on molecular methods, especially "nested PCR". There are various methods for the isolation of DNA from leaf-stalks and the phloem (from stalk or roots), and four different methods weretested: Lee et al. (1991), two commercial DNA isolation kits (DNeasy Plant Maxi Kit and NucleoSpin; Plant XL) and, as a comparison, the method described by Ahrens and Seemüller (1992). For the nested PCR detection of ESFY we used non-specific primers like R16F1/R16R0 (out-primers) and fU5/rU3 (in-primers). The results demonstrate that none of the methods used for the DNA isolation and subsequent PCR reactions provide the consistent quality and quantity of DNA which is necessary for ESFY

Název v anglickém jazyce

Comparison of Methods of Isolating DNA for ESFY Phytoplasma Detection

Popis výsledku anglicky

'Candidatus phytoplasma prunorum', (European stone fruit yellows phytoplasma - ESFY), is nowadays one of the most important diseases belonging to the list of organisms for which quarantine is required, and is a long-standing problem in apricot and peachorchards in the Czech Republic. Its detection and diagnosis is based on molecular methods, especially "nested PCR". There are various methods for the isolation of DNA from leaf-stalks and the phloem (from stalk or roots), and four different methods weretested: Lee et al. (1991), two commercial DNA isolation kits (DNeasy Plant Maxi Kit and NucleoSpin; Plant XL) and, as a comparison, the method described by Ahrens and Seemüller (1992). For the nested PCR detection of ESFY we used non-specific primers like R16F1/R16R0 (out-primers) and fU5/rU3 (in-primers). The results demonstrate that none of the methods used for the DNA isolation and subsequent PCR reactions provide the consistent quality and quantity of DNA which is necessary for ESFY

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta horticulturae

ISSN

0567-7572

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

825

Stát vydavatele periodika

BE - Belgické království

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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