The Evaluation of Virus Symptoms and Fruit Quality of GMO, PPV-Resistant P. domestica 'HoneySweet' Grown in the Open Field under a High and Permanent Infection Pressure of PPV, ACLSV, and PDV

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F13%3A00216015" target="_blank" >RIV/62156489:43510/13:00216015 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The Evaluation of Virus Symptoms and Fruit Quality of GMO, PPV-Resistant P. domestica 'HoneySweet' Grown in the Open Field under a High and Permanent Infection Pressure of PPV, ACLSV, and PDV

Popis výsledku v původním jazyce

Transgenic, 'HoneySweet' plums inoculated with Plum pox virus, recombinant strain (PPV-Rec) alone, PPV-Rec + Apple chlorotic leafspot virus (ACLSV), PPV-Rec + Prune dwarf virus (PDV), and PPV-Rec + ACLSV + PDV were grown in an open field under high and permanent infection pressure both from graft inoculation and natural aphid vectors for 8 years. 'HoneySweet' control trees were non-graft-inoculated trees. Plants were monitored by symptomology, DAS-ELISA, semiquantitative (SQ)-ELISA, and real-time (RT)-PCR. Inoculum shoots showed severe PPV symptoms from the first year after inoculation throughout the study. Mild PPV symptoms appeared in some of the basal leaves of graft-inoculated 'HoneySweet' trees in the second year following inoculation. During thefollowing six years, even these mild and isolated symptoms on 'HoneySweet' trees diminished. The presence of PPV in these basal areas of 'HoneySweet' trees was confirmed by ELISA, ISEM, and RT-PCR. Relative concentration of PPV determined

Název v anglickém jazyce

The Evaluation of Virus Symptoms and Fruit Quality of GMO, PPV-Resistant P. domestica 'HoneySweet' Grown in the Open Field under a High and Permanent Infection Pressure of PPV, ACLSV, and PDV

Popis výsledku anglicky

Transgenic, 'HoneySweet' plums inoculated with Plum pox virus, recombinant strain (PPV-Rec) alone, PPV-Rec + Apple chlorotic leafspot virus (ACLSV), PPV-Rec + Prune dwarf virus (PDV), and PPV-Rec + ACLSV + PDV were grown in an open field under high and permanent infection pressure both from graft inoculation and natural aphid vectors for 8 years. 'HoneySweet' control trees were non-graft-inoculated trees. Plants were monitored by symptomology, DAS-ELISA, semiquantitative (SQ)-ELISA, and real-time (RT)-PCR. Inoculum shoots showed severe PPV symptoms from the first year after inoculation throughout the study. Mild PPV symptoms appeared in some of the basal leaves of graft-inoculated 'HoneySweet' trees in the second year following inoculation. During thefollowing six years, even these mild and isolated symptoms on 'HoneySweet' trees diminished. The presence of PPV in these basal areas of 'HoneySweet' trees was confirmed by ELISA, ISEM, and RT-PCR. Relative concentration of PPV determined

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QI101A123" target="_blank" >QI101A123: Komplexní výzkum rezistence transgenních rostlin Prunus domestica L., klon C5 k viru šarky švestky, viru zakrslosti slivoně a viru chlorotické skvrnitosti jabloně, identifikace netransgenních zdrojů rezistence slivoně k PPV.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae 974

ISBN

978-90-66050-97-6

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

65-70

Název nakladatele

International Society for Horticultural Science

Místo vydání

Leuven

Místo konání akce

White River, South Africa

Datum konání akce

11. 9. 2011

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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