An evaluation of the impact of demethylating agents treatment using TGS 16C nicotiana benthamiana reporter line

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F15%3A43906742" target="_blank" >RIV/62156489:43510/15:43906742 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

An evaluation of the impact of demethylating agents treatment using TGS 16C nicotiana benthamiana reporter line

Popis výsledku v původním jazyce

Epigenetics is one of the fastest-growing areas of science and has now become a central issue in biological studies of development, stress impact and disease. DNA methylation provides a way how to alter the gene expression pattern without disrupting or modifying the genome. Here, we demonstrate the potential of a Nicotiana benthamianaTGS 16C reporter line in the signalization of demethylating events caused by the activity of the so-called demethylating agents. These compounds have the ability to block or to interfere with the activity of methyltransferases, enzymes responsible for maintaining methylation marks on the replicating sequences. This N. benthamianaline carries a green fluorescent protein gene (GFP), whose promoter had been methylated and isthus inactive. By treating such plants with compounds with demethylating properties, their demethylating potential can be estimated by the effect on the re-established GFP expression in plant tissues.

Název v anglickém jazyce

An evaluation of the impact of demethylating agents treatment using TGS 16C nicotiana benthamiana reporter line

Popis výsledku anglicky

Epigenetics is one of the fastest-growing areas of science and has now become a central issue in biological studies of development, stress impact and disease. DNA methylation provides a way how to alter the gene expression pattern without disrupting or modifying the genome. Here, we demonstrate the potential of a Nicotiana benthamianaTGS 16C reporter line in the signalization of demethylating events caused by the activity of the so-called demethylating agents. These compounds have the ability to block or to interfere with the activity of methyltransferases, enzymes responsible for maintaining methylation marks on the replicating sequences. This N. benthamianaline carries a green fluorescent protein gene (GFP), whose promoter had been methylated and isthus inactive. By treating such plants with compounds with demethylating properties, their demethylating potential can be estimated by the effect on the re-established GFP expression in plant tissues.

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2015: Proceedings of International PhD Students Conference

ISBN

978-80-7509-363-9

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

428-433

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

11. 11. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000366466100080