Evaluation of different methods of dna extraction for detection of bacterium Xanthomonas campestris pv. Campestris in cabbage leaves
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F15%3A43907066" target="_blank" >RIV/62156489:43510/15:43907066 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.acta.media.pl/pl/full/7/2015/000070201500014000060014100150.pdf" target="_blank" >http://www.acta.media.pl/pl/full/7/2015/000070201500014000060014100150.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Evaluation of different methods of dna extraction for detection of bacterium Xanthomonas campestris pv. Campestris in cabbage leaves
Popis výsledku v původním jazyce
The bacterium Xanthomonas campestris pathovar campestris (Xcc) as the causal agent of black rot of cruciferous plants causes considerable losses in agricultural yield all over the world. The control of black rot is difficult as well as the determinationof Xcc on the basis of morphological parameters or by pathogenicity testing. Ten different possibilities for extraction bacterial DNA followed by PCR detection method were tested to optimize PCR protocol. On the basis of ISTA validated method, three setsof primers UBP 1052F-BACR, DLH 120-125 and ZUP 2309-2310 were used. The results of measured concentration and quality of DNA and efficacy for PCR amplification were compared. Finally, three approaches for DNA extraction within Xanthomonas campestris pv.campestris detection protocol were recommended - commercial kits used for isolation from tissues by Macherey-Nagel and MO BIO and kit intended for microbial cultures by MO BIO.
Název v anglickém jazyce
Evaluation of different methods of dna extraction for detection of bacterium Xanthomonas campestris pv. Campestris in cabbage leaves
Popis výsledku anglicky
The bacterium Xanthomonas campestris pathovar campestris (Xcc) as the causal agent of black rot of cruciferous plants causes considerable losses in agricultural yield all over the world. The control of black rot is difficult as well as the determinationof Xcc on the basis of morphological parameters or by pathogenicity testing. Ten different possibilities for extraction bacterial DNA followed by PCR detection method were tested to optimize PCR protocol. On the basis of ISTA validated method, three setsof primers UBP 1052F-BACR, DLH 120-125 and ZUP 2309-2310 were used. The results of measured concentration and quality of DNA and efficacy for PCR amplification were compared. Finally, three approaches for DNA extraction within Xanthomonas campestris pv.campestris detection protocol were recommended - commercial kits used for isolation from tissues by Macherey-Nagel and MO BIO and kit intended for microbial cultures by MO BIO.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
GE - Šlechtění rostlin
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/LH12196" target="_blank" >LH12196: Rozvoj metod a studia stresového metobolismu a genomiky v Číně a České republice</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Acta Scientiarum Polonorum. Hortorum Cultus
ISSN
1644-0692
e-ISSN
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Svazek periodika
14
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
PL - Polská republika
Počet stran výsledku
10
Strana od-do
141-150
Kód UT WoS článku
000365962000010
EID výsledku v databázi Scopus
2-s2.0-84949229042