Spatial and temporal variation of active fungal communities on grapevine propagating material after hot-water treatment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F17%3A43912419" target="_blank" >RIV/62156489:43510/17:43912419 - isvavai.cz</a>

Výsledek na webu

<a href="http://managtd.eu/images/uploads/content/257/Abstracts%2010th%20IWGTD%20Reims%202017.pdf" target="_blank" >http://managtd.eu/images/uploads/content/257/Abstracts%2010th%20IWGTD%20Reims%202017.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Spatial and temporal variation of active fungal communities on grapevine propagating material after hot-water treatment

Popis výsledku v původním jazyce

Grapevine propagating material is particularly vulnerable to infections caused by fungal trunk disease (TD) pathogens in nurseries since many wounds and cuts are made during the propagation process. Hotwater treatment (HWT) is the only currently recognized means of controlling these internal infections. The objective of this study was to examine the effect of HWT on the total active mycoflora, including fungal TD pathogens. For this purpose, two experiments were simultaneously carried out in Spain and the Czech Republic. Dormant grafted plants were hotwater treated at 50 or 53oC for 30 min. In April 2015, HWTed vines were planted immediately in vineyards, following standard cultural practices in each country. At the end of the growing season (October 2015), plants were uprooted and evaluated. Fungal communities were analyzed at two periods: i) immediately after HWT; and ii) after one growing season for a total of 480 grafted plants. For each plant, tissue samples from the root and graft union were analyzed by classical isolation and fungal identification, and high throughput metatranscriptomic sequencing of the ITS2 region. The results revealed significant differences in the detection levels of both methods. The metatranscriptomic sequencing increased the resolution of the fungal community analysis and revealed a highly diverse and complex mycoflora of grapevine wood compared to the classical approach. In classical isolation approach, the total active mycoflora, including fungal TD pathogens, was reduced immediately after HWT. However, the number of fungal colonies increased after one growing season. The results demonstrate that the HWT process does not completely sterilize the internal wood of grapevine propagating materials. This represents the first study on active fungal communities on grapevine grafted plants using metatranscriptomics approach in combination with classical isolation. Furthermore, it provides important and practical useful insights on the dynamics of the active fungal communities of HWTed plants.

Název v anglickém jazyce

Spatial and temporal variation of active fungal communities on grapevine propagating material after hot-water treatment

Popis výsledku anglicky

Grapevine propagating material is particularly vulnerable to infections caused by fungal trunk disease (TD) pathogens in nurseries since many wounds and cuts are made during the propagation process. Hotwater treatment (HWT) is the only currently recognized means of controlling these internal infections. The objective of this study was to examine the effect of HWT on the total active mycoflora, including fungal TD pathogens. For this purpose, two experiments were simultaneously carried out in Spain and the Czech Republic. Dormant grafted plants were hotwater treated at 50 or 53oC for 30 min. In April 2015, HWTed vines were planted immediately in vineyards, following standard cultural practices in each country. At the end of the growing season (October 2015), plants were uprooted and evaluated. Fungal communities were analyzed at two periods: i) immediately after HWT; and ii) after one growing season for a total of 480 grafted plants. For each plant, tissue samples from the root and graft union were analyzed by classical isolation and fungal identification, and high throughput metatranscriptomic sequencing of the ITS2 region. The results revealed significant differences in the detection levels of both methods. The metatranscriptomic sequencing increased the resolution of the fungal community analysis and revealed a highly diverse and complex mycoflora of grapevine wood compared to the classical approach. In classical isolation approach, the total active mycoflora, including fungal TD pathogens, was reduced immediately after HWT. However, the number of fungal colonies increased after one growing season. The results demonstrate that the HWT process does not completely sterilize the internal wood of grapevine propagating materials. This represents the first study on active fungal communities on grapevine grafted plants using metatranscriptomics approach in combination with classical isolation. Furthermore, it provides important and practical useful insights on the dynamics of the active fungal communities of HWTed plants.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/LD14051" target="_blank" >LD14051: Vývoj spolehlivého systému pro detekci a kvantifikaci patogenů způsobujících onemocnění kmínků révy a mapování výskytu tohoto onemocnění v ČR</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů