In vitro inducted tetraploid Petunia hybrida of a red color

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F17%3A43912587" target="_blank" >RIV/62156489:43510/17:43912587 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

In vitro inducted tetraploid Petunia hybrida of a red color

Popis výsledku v původním jazyce

Petunia hybrida is one of the most important annuals. Niche of the assortment represent a group of tetraploid varieties that are interesting due to their giant flower. However, these are only old open pollinated varieties. Creation of homozygous tetraploid lines is a prerequisite for making F1 varieties. To do so, two homozygous lines used in commercial production of F1 varieties were used. 10 days old seedlings grown on MS medium with 8 g/l Agar and 20 g/l sucrose were treated with a solution of 30 μM oryzalin for 24 hours in in vitro conditions. The plants were washed and transplanted to the same medium. Later, they were grown in the greenhouse. Cytometrically, ploidy was detected. The yield of tetraploids was more than 50%. Octoploid plants were not found. Tetraploid red flowering plants with genotype GGGG and gggg were obtained. Flower size increased by at least 30% for polyploidy.

Název v anglickém jazyce

In vitro inducted tetraploid Petunia hybrida of a red color

Popis výsledku anglicky

Petunia hybrida is one of the most important annuals. Niche of the assortment represent a group of tetraploid varieties that are interesting due to their giant flower. However, these are only old open pollinated varieties. Creation of homozygous tetraploid lines is a prerequisite for making F1 varieties. To do so, two homozygous lines used in commercial production of F1 varieties were used. 10 days old seedlings grown on MS medium with 8 g/l Agar and 20 g/l sucrose were treated with a solution of 30 μM oryzalin for 24 hours in in vitro conditions. The plants were washed and transplanted to the same medium. Later, they were grown in the greenhouse. Cytometrically, ploidy was detected. The yield of tetraploids was more than 50%. Octoploid plants were not found. Tetraploid red flowering plants with genotype GGGG and gggg were obtained. Flower size increased by at least 30% for polyploidy.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2017: Proceedings of International PhD Students Conference

ISBN

978-80-7509-529-9

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

5

Strana od-do

613-617

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

8. 11. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000440194500109