High-throughput small RNA sequencing for evaluation of grapevine sanitation efficacy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F19%3A43915562" target="_blank" >RIV/62156489:43510/19:43915562 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027006:_____/19:00005100

Výsledek na webu

<a href="https://doi.org/10.1016/j.jviromet.2019.03.003" target="_blank" >https://doi.org/10.1016/j.jviromet.2019.03.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jviromet.2019.03.003" target="_blank" >10.1016/j.jviromet.2019.03.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

High-throughput small RNA sequencing for evaluation of grapevine sanitation efficacy

Popis výsledku v původním jazyce

This study describes the application of high-throughput sequencing of small RNA analysis of the efficacy of using Ribavirin to eliminate Grapevine leafroll-associated virus 1, Grapevine fleck virus and Grapevine rupestris stem pitting-associated virus from Vitis vinifera cv. Riesling. The original plant used for sanitation by Ribavirin treatment was one naturally infected with all the viruses mentioned above as confirmed by RT-PCR. A tissue cultures of the plant were established and plantlets obtained were sanitized using Ribavirin. Three years after sanitation, a small RNA sequencing method for virus detection, targeting 21, 22 and 24 nt-long viral small RNAs (vsRNAs), was used to analyze both the mother plant and the sanitized plants. The results showed that the mother plant was infected by the three mentioned viruses and additionally by two viroids - Hop stunt viroid and Grapevine yellow speckle viroid 1. After Ribavirin treatment, the plants contained only the two viroids, with the complete elimination of all the viruses previously present.

Název v anglickém jazyce

High-throughput small RNA sequencing for evaluation of grapevine sanitation efficacy

Popis výsledku anglicky

This study describes the application of high-throughput sequencing of small RNA analysis of the efficacy of using Ribavirin to eliminate Grapevine leafroll-associated virus 1, Grapevine fleck virus and Grapevine rupestris stem pitting-associated virus from Vitis vinifera cv. Riesling. The original plant used for sanitation by Ribavirin treatment was one naturally infected with all the viruses mentioned above as confirmed by RT-PCR. A tissue cultures of the plant were established and plantlets obtained were sanitized using Ribavirin. Three years after sanitation, a small RNA sequencing method for virus detection, targeting 21, 22 and 24 nt-long viral small RNAs (vsRNAs), was used to analyze both the mother plant and the sanitized plants. The results showed that the mother plant was infected by the three mentioned viruses and additionally by two viroids - Hop stunt viroid and Grapevine yellow speckle viroid 1. After Ribavirin treatment, the plants contained only the two viroids, with the complete elimination of all the viruses previously present.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Virological Methods

ISSN

0166-0934

e-ISSN

—

Svazek periodika

267

Číslo periodika v rámci svazku

May

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

66-70

Kód UT WoS článku

000465365600011

EID výsledku v databázi Scopus

2-s2.0-85062734452