Grapevine Trunk Diseases Pathogens Identification on Grapevine Rootstocks in Ukraine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F19%3A43916713" target="_blank" >RIV/62156489:43510/19:43916713 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.15407/microbiolj81.02.065" target="_blank" >https://doi.org/10.15407/microbiolj81.02.065</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15407/microbiolj81.02.065" target="_blank" >10.15407/microbiolj81.02.065</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Grapevine Trunk Diseases Pathogens Identification on Grapevine Rootstocks in Ukraine

Popis výsledku v původním jazyce

The objective of this study was isolation of fungi from the grapevine trunks, DNA extraction and identification of potential esca pathogens in Ukraine. Methods. Isolation and identification of fungal cultures based on morphology and DNA sequencing. Total DNA of fungal colonies was extracted by NucleoSpin Tissue kit (Macherey-Nagel, Düren, Germany) according to manufacturer&apos;s instructions. To amplify ITS region, ITS1 and ITS4 primers were used. The PCR products corresponding to the size of approx. 550 bp. were sequenced and the obtained nucleotide sequences were analysed using CLC Main Workbench 5.0 (CLC bio, Aarhus, Denmark). Results. Determination of potential pathogens complex showed the presence of species associated with &quot;trunk diseases&quot;. Among specific esca pathogens 3 isolates of Cadophora luteo-olivacea were found out. Esca-affected samples also showed the presence of Eutypa lata (causal agent of Eutypa dieback) and Botryosphaeria dothidea, (causal agent of Botrioshaeria dieback). Conclusions. DNA identification of fungal pathogens showed a discrepancy in species composition between asymptomatic plants and esca-affected plants. Esca-affected cultivars showed the presence of Eutypa lata and Botryosphaeria dothidea, which were absent in asymptomatic plants.

Název v anglickém jazyce

Grapevine Trunk Diseases Pathogens Identification on Grapevine Rootstocks in Ukraine

Popis výsledku anglicky

The objective of this study was isolation of fungi from the grapevine trunks, DNA extraction and identification of potential esca pathogens in Ukraine. Methods. Isolation and identification of fungal cultures based on morphology and DNA sequencing. Total DNA of fungal colonies was extracted by NucleoSpin Tissue kit (Macherey-Nagel, Düren, Germany) according to manufacturer&apos;s instructions. To amplify ITS region, ITS1 and ITS4 primers were used. The PCR products corresponding to the size of approx. 550 bp. were sequenced and the obtained nucleotide sequences were analysed using CLC Main Workbench 5.0 (CLC bio, Aarhus, Denmark). Results. Determination of potential pathogens complex showed the presence of species associated with &quot;trunk diseases&quot;. Among specific esca pathogens 3 isolates of Cadophora luteo-olivacea were found out. Esca-affected samples also showed the presence of Eutypa lata (causal agent of Eutypa dieback) and Botryosphaeria dothidea, (causal agent of Botrioshaeria dieback). Conclusions. DNA identification of fungal pathogens showed a discrepancy in species composition between asymptomatic plants and esca-affected plants. Esca-affected cultivars showed the presence of Eutypa lata and Botryosphaeria dothidea, which were absent in asymptomatic plants.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mìkrobiologičnij Žurnal

ISSN

1028-0987

e-ISSN

—

Svazek periodika

81

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

UA - Ukrajina

Počet stran výsledku

8

Strana od-do

65-72

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85075170209