Medium-term in vitro storage of vegetative propagated genotypes of Petunia hybrida and Calibrachoa in minimal amount of media

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F19%3A43917090" target="_blank" >RIV/62156489:43510/19:43917090 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Medium-term in vitro storage of vegetative propagated genotypes of Petunia hybrida and Calibrachoa in minimal amount of media

Popis výsledku v původním jazyce

In vitro storage of genetic materials is crucial for multiplication and breeding reasons. It prevents loosing valuable genotypes, which can happen due to biotic and abiotic effects while in vivo planting. Conventional in vitro storage of Petunia hybrida and Calibrachoa genotypes is labor and cost effective. Three explants are usually cultivated in one glass Erlenmeyer flask with MS Cultivation medium with 20 g/l saccharoses and 6 g/l agar added. Passaging needs to be done four times a year. The aim of this experiment was to determine a way how to store genotypes of vegetative propagated Petunia hybrida (diploid and tetraploid materials) and Calibrachoa in the conditions, so only one passaging will be needed. Stored material must regenerate fast, so it can be used promptly in breeding programs or experiments.

Název v anglickém jazyce

Medium-term in vitro storage of vegetative propagated genotypes of Petunia hybrida and Calibrachoa in minimal amount of media

Popis výsledku anglicky

In vitro storage of genetic materials is crucial for multiplication and breeding reasons. It prevents loosing valuable genotypes, which can happen due to biotic and abiotic effects while in vivo planting. Conventional in vitro storage of Petunia hybrida and Calibrachoa genotypes is labor and cost effective. Three explants are usually cultivated in one glass Erlenmeyer flask with MS Cultivation medium with 20 g/l saccharoses and 6 g/l agar added. Passaging needs to be done four times a year. The aim of this experiment was to determine a way how to store genotypes of vegetative propagated Petunia hybrida (diploid and tetraploid materials) and Calibrachoa in the conditions, so only one passaging will be needed. Stored material must regenerate fast, so it can be used promptly in breeding programs or experiments.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2019: Proceedings of International PhD Students Conference

ISBN

978-80-7509-688-3

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

409-413

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

6. 11. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000576735500073