First report of Grapevine Pinot gris virus on grapevines in Armenia

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F20%3A43916853" target="_blank" >RIV/62156489:43510/20:43916853 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1094/PDIS-09-19-1944-PDN" target="_blank" >https://doi.org/10.1094/PDIS-09-19-1944-PDN</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1094/PDIS-09-19-1944-PDN" target="_blank" >10.1094/PDIS-09-19-1944-PDN</a>

Jazyk výsledku

angličtina

Název v původním jazyce

First report of Grapevine Pinot gris virus on grapevines in Armenia

Popis výsledku v původním jazyce

Grapevine Pinot gris virus (GPGV) is a member of the genus Trichovirus in the family Betaflexiviridae. It was first described on Italian grapevine (Vitis vinifera L.) cv. Pinot gris in which it provoked a disease known as grapevine leaf mottling and deformation (GLMD) (Giampetruzzi et al. 2012; Saldarelli et al. 2017). Studies on GPGV revealed a widespread occurrence of GPGV in many wine-producing countries in the world (Eichmeier et al. 2018) and more recently, in Canada (Poojari et al. 2016), Australia (Wu and Habili 2017), Brazil (Fajadro et al. 2017), Chile (Zamorano et al. 2019), Pakistan (Rasool et al. 2018) and the UK (Silva et al. 2018). In 2018, 17 grapevines cvs. Ararati, Arevabuyr, Arevar, Armenia, Eraskheni, Garan Dmak, Itsaptuk, Kishmish White, Masis, Meghrabuyr, Mskhali, Rkatsiteli, Shahumyani, Tokun, Vani, Vardaguyn Yerevani and Voskehat were selected in economically important Armenian vineyards of Yerevan city and Merzdavan village for GPGV detection. The grapevines were selected based on possible GLMD symptoms (Fig. 1). The plants were tested by reverse transcription polymerase chain reaction (RT-PCR) for GPGV presence as described by Eichmeier et al. (2018). GPGV was detected in cvs. Ararati, Arevabuyr, Eraskheni, Garan Dmak, Itsaptuk and Mskhali based on the amplification of gene fragments specific to the MP-CP and RdRp genome regions (Eichmeier et al. 2018). The derived sequences were submitted into GenBank under Acc. Nos. MN186552-MN186556 (MP-CP) and MN186557-MN186561 (RdRp). To obtain more genome sequence from a selected GLMD symptomatic isolate cv Eraskheni (Fig. 1), small RNA high-throughput sequencing (HTS) was used. Total RNA was extracted from cambial scrapings using TRIzolTM Reagent (TFS, Waltham, USA), and used for constructing a cDNA library (TruSeq Small RNA Library Prep Kit, Illumina, San Diego, USA) followed by sequencing (1x36 bp) on an Illumina MiniSeq sequencer using MiniSeq High Output Reagent Kit (75-cycles) (Illumina). Bioinformatic evaluation was done as described by Eichmeier et al. (2019). HTS revealed the presence of GPGV (one contig; length: 5,535 bp, number of mapped reads: 299, mean read coverage: 1.08, genome coverage: 76.25%, identity with ref. seq. NC_015782: 99.06%), in mixed infection with Grapevine fleck virus, Grapevine leafroll-associated virus 1, Grapevine fanleaf virus and Grapevine virus A in the sample. The genome sequence of GPGV cv. Eraskheni was deposited under Acc. No. MN186562. Our preliminary data showed a high prevalence of GPGV, roughly 35%, in Armenia. The genetic diversity and the impact of this virus on grapevine in Armenia should be studied further.

Název v anglickém jazyce

First report of Grapevine Pinot gris virus on grapevines in Armenia

Popis výsledku anglicky

Grapevine Pinot gris virus (GPGV) is a member of the genus Trichovirus in the family Betaflexiviridae. It was first described on Italian grapevine (Vitis vinifera L.) cv. Pinot gris in which it provoked a disease known as grapevine leaf mottling and deformation (GLMD) (Giampetruzzi et al. 2012; Saldarelli et al. 2017). Studies on GPGV revealed a widespread occurrence of GPGV in many wine-producing countries in the world (Eichmeier et al. 2018) and more recently, in Canada (Poojari et al. 2016), Australia (Wu and Habili 2017), Brazil (Fajadro et al. 2017), Chile (Zamorano et al. 2019), Pakistan (Rasool et al. 2018) and the UK (Silva et al. 2018). In 2018, 17 grapevines cvs. Ararati, Arevabuyr, Arevar, Armenia, Eraskheni, Garan Dmak, Itsaptuk, Kishmish White, Masis, Meghrabuyr, Mskhali, Rkatsiteli, Shahumyani, Tokun, Vani, Vardaguyn Yerevani and Voskehat were selected in economically important Armenian vineyards of Yerevan city and Merzdavan village for GPGV detection. The grapevines were selected based on possible GLMD symptoms (Fig. 1). The plants were tested by reverse transcription polymerase chain reaction (RT-PCR) for GPGV presence as described by Eichmeier et al. (2018). GPGV was detected in cvs. Ararati, Arevabuyr, Eraskheni, Garan Dmak, Itsaptuk and Mskhali based on the amplification of gene fragments specific to the MP-CP and RdRp genome regions (Eichmeier et al. 2018). The derived sequences were submitted into GenBank under Acc. Nos. MN186552-MN186556 (MP-CP) and MN186557-MN186561 (RdRp). To obtain more genome sequence from a selected GLMD symptomatic isolate cv Eraskheni (Fig. 1), small RNA high-throughput sequencing (HTS) was used. Total RNA was extracted from cambial scrapings using TRIzolTM Reagent (TFS, Waltham, USA), and used for constructing a cDNA library (TruSeq Small RNA Library Prep Kit, Illumina, San Diego, USA) followed by sequencing (1x36 bp) on an Illumina MiniSeq sequencer using MiniSeq High Output Reagent Kit (75-cycles) (Illumina). Bioinformatic evaluation was done as described by Eichmeier et al. (2019). HTS revealed the presence of GPGV (one contig; length: 5,535 bp, number of mapped reads: 299, mean read coverage: 1.08, genome coverage: 76.25%, identity with ref. seq. NC_015782: 99.06%), in mixed infection with Grapevine fleck virus, Grapevine leafroll-associated virus 1, Grapevine fanleaf virus and Grapevine virus A in the sample. The genome sequence of GPGV cv. Eraskheni was deposited under Acc. No. MN186562. Our preliminary data showed a high prevalence of GPGV, roughly 35%, in Armenia. The genetic diversity and the impact of this virus on grapevine in Armenia should be studied further.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů