The use of SSRs for the identification of unknown Asian pear cultivars
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F21%3A43919605" target="_blank" >RIV/62156489:43510/21:43919605 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.17660/ActaHortic.2021.1307.32" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2021.1307.32</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17660/ActaHortic.2021.1307.32" target="_blank" >10.17660/ActaHortic.2021.1307.32</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The use of SSRs for the identification of unknown Asian pear cultivars
Popis výsledku v původním jazyce
Pear species belong to one of the longest-cultivated temperate species. A large number of cultivars is spread worldwide, that have different names in different countries. Probably the most famous is the cultivar 'Williams', which is known in the USA as 'Bartlett'. However, not all synonyms are always known. Molecular methods, namely the SSR method, allow reliable identification of cultivars. In this study, an SSR marker set was applied to 31 Asian pear cultivars, 4 interspecific hybrids and 3 botanical species from the East China gene center. Six SSR primer pairs were used to determine the genetic relatedness. SSR primers were labeled with fluorescent dyes and the PCR products were evaluated using a genetic analyzer. Based on the obtained results, the cultivars were divided into two main clusters. Cluster I was divided into three subclusters, subcluster I contained P. pyrifolia and cultivars derived therefrom, subcluster II contained P. ussuriensis and several ussuriensis-type cultivars and Chinese white pear cultivars and subcluster III contained cultivars of the Chinese white pears. Cluster II was formed by only 4 cultivars probably being interspecific hybrids. Further, combinations of cultivars 'Man San Gill' and 'Pung Su'; 'Early' and 'Zaosu Li'; 'Snow Flower', 'Shon Shu' and 'Dangshanshu Li' had identical SSR profiles, which indicates, that they are probably identical cultivars or possibly different clones.
Název v anglickém jazyce
The use of SSRs for the identification of unknown Asian pear cultivars
Popis výsledku anglicky
Pear species belong to one of the longest-cultivated temperate species. A large number of cultivars is spread worldwide, that have different names in different countries. Probably the most famous is the cultivar 'Williams', which is known in the USA as 'Bartlett'. However, not all synonyms are always known. Molecular methods, namely the SSR method, allow reliable identification of cultivars. In this study, an SSR marker set was applied to 31 Asian pear cultivars, 4 interspecific hybrids and 3 botanical species from the East China gene center. Six SSR primer pairs were used to determine the genetic relatedness. SSR primers were labeled with fluorescent dyes and the PCR products were evaluated using a genetic analyzer. Based on the obtained results, the cultivars were divided into two main clusters. Cluster I was divided into three subclusters, subcluster I contained P. pyrifolia and cultivars derived therefrom, subcluster II contained P. ussuriensis and several ussuriensis-type cultivars and Chinese white pear cultivars and subcluster III contained cultivars of the Chinese white pears. Cluster II was formed by only 4 cultivars probably being interspecific hybrids. Further, combinations of cultivars 'Man San Gill' and 'Pung Su'; 'Early' and 'Zaosu Li'; 'Snow Flower', 'Shon Shu' and 'Dangshanshu Li' had identical SSR profiles, which indicates, that they are probably identical cultivars or possibly different clones.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
—
OECD FORD obor
40105 - Horticulture, viticulture
Návaznosti výsledku
Projekt
<a href="/cs/project/QK1910137" target="_blank" >QK1910137: Využití nových ovocných druhů pro dlouhodobé udržení produkčního potenciálu ovocných výsadeb v podmínkách měnícího se klimatu</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
Acta Horticulturae 1307
ISBN
978-94-6261-306-5
ISSN
0567-7572
e-ISSN
2406-6168
Počet stran výsledku
7
Strana od-do
205-211
Název nakladatele
International Society for Horticultural Science (ISHS)
Místo vydání
Leuven
Místo konání akce
Praha
Datum konání akce
3. 6. 2019
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
000948137200031