Electrotherapy; a promising therapy to eradicate viruses from infected in vitro olive cv. Meshkat

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F24%3A43925212" target="_blank" >RIV/62156489:43510/24:43925212 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/s11240-024-02777-7" target="_blank" >https://doi.org/10.1007/s11240-024-02777-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-024-02777-7" target="_blank" >10.1007/s11240-024-02777-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrotherapy; a promising therapy to eradicate viruses from infected in vitro olive cv. Meshkat

Popis výsledku v původním jazyce

Olive tree harbors several viruses affecting the yield and quality of fruit worldwide. Application of virus-free planting materials is one of the main strategies to counteract virus diseases. The elimination of Arabis mosaic virus (ArMV), Cherry leaf roll virus (CLRV), Cucumber mosaic virus (CMV) and Strawberry latent ringspot virus (SLRSV) by different regimes of electrotherapy followed by 1 mm shoot tip culture was investigated in a native Iranian olive cultivar Meshkat. The results showed that survival, regrowth and proliferation rates were dependent on the therapy regime and shoot tip type. Apical shoot tips showed more significant proliferation rate compared to axillary ones under 30 min treatment of electric current. In addition, apical shoot tips represented higher survival and regrowth rate under 30 min of electrotherapy, 54 and 46% respectively compared to 15 min of therapy. Interestingly, the regrowth rate in the virus mixed-infected cv. Meshkat was notably increased by increasing the electrotherapy duration from 13 min to 10% to 40 min and 46% in axillary and apical shoot tips respectively. The traditional method of shoot tip culture has not been able to eradicate the viruses in question. However, ArMV, CLRV, CMV and SLRSV were completely eradicated by electrotherapy treatment (35 mA, 100 V, 30 min) and the therapy efficiency index (TEI) was 54 for all studied viruses. Moreover, electrotherapy treatment (35 mA, 100 V, 15 min) eradicated only ArMV (62.5%) and CLRV (50%) with TEI of 31.25 and 25 respectively. The results concluded that the combination of electrotherapy with shoot tip culture can be successfully used as a virus elimination method for producing virus-free olive plants.

Název v anglickém jazyce

Electrotherapy; a promising therapy to eradicate viruses from infected in vitro olive cv. Meshkat

Popis výsledku anglicky

Olive tree harbors several viruses affecting the yield and quality of fruit worldwide. Application of virus-free planting materials is one of the main strategies to counteract virus diseases. The elimination of Arabis mosaic virus (ArMV), Cherry leaf roll virus (CLRV), Cucumber mosaic virus (CMV) and Strawberry latent ringspot virus (SLRSV) by different regimes of electrotherapy followed by 1 mm shoot tip culture was investigated in a native Iranian olive cultivar Meshkat. The results showed that survival, regrowth and proliferation rates were dependent on the therapy regime and shoot tip type. Apical shoot tips showed more significant proliferation rate compared to axillary ones under 30 min treatment of electric current. In addition, apical shoot tips represented higher survival and regrowth rate under 30 min of electrotherapy, 54 and 46% respectively compared to 15 min of therapy. Interestingly, the regrowth rate in the virus mixed-infected cv. Meshkat was notably increased by increasing the electrotherapy duration from 13 min to 10% to 40 min and 46% in axillary and apical shoot tips respectively. The traditional method of shoot tip culture has not been able to eradicate the viruses in question. However, ArMV, CLRV, CMV and SLRSV were completely eradicated by electrotherapy treatment (35 mA, 100 V, 30 min) and the therapy efficiency index (TEI) was 54 for all studied viruses. Moreover, electrotherapy treatment (35 mA, 100 V, 15 min) eradicated only ArMV (62.5%) and CLRV (50%) with TEI of 31.25 and 25 respectively. The results concluded that the combination of electrotherapy with shoot tip culture can be successfully used as a virus elimination method for producing virus-free olive plants.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40105 - Horticulture, viticulture

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell, Tissue and Organ Culture

ISSN

0167-6857

e-ISSN

1573-5044

Svazek periodika

157

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

52

Kód UT WoS článku

001227627200002

EID výsledku v databázi Scopus

2-s2.0-85193613351