Porcine obese (leptin) and growth hormone gene: frequency of genotypes and alleles in Duroc and hybrid pigs

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Porcine obese (leptin) and growth hormone gene: frequency of genotypes and alleles in Duroc and hybrid pigs

Popis výsledku v původním jazyce

The objective ot the present study was to compare distribution of the genotypes and alleles of growth hormone (GH) and obese (Lep) genes and analyse possible interaction between them. The genotypes of the analysed genes were determined by PCR-RFLP usingHaeII, MspI (growth hormone gene) and HinfI restriction endonucleases ( obese gene) in 115 Duroc and 180 hybrid pigs. The alleles were characterised with these fragments: GH- HaeII: allele - = 506bp; allele + = 333, 173bp; GH-MspI: allele - = 284, 222bp;allele + = 222, 147, 137bp; Lep: allele T = 152bp, allele C = 84, 68bp. Frequencies of genotypes of all involved genes in both populations showed a concordant distribution of genotypes and were in a genetic equilibrium according to the Hardy-Weinberg law. We found the statistically significantly different GH-HaeII and Lep gene distribution between breed Duroc and hybrid pigs.

Název v anglickém jazyce

Porcine obese (leptin) and growth hormone gene: frequency of genotypes and alleles in Duroc and hybrid pigs

Popis výsledku anglicky

The objective ot the present study was to compare distribution of the genotypes and alleles of growth hormone (GH) and obese (Lep) genes and analyse possible interaction between them. The genotypes of the analysed genes were determined by PCR-RFLP usingHaeII, MspI (growth hormone gene) and HinfI restriction endonucleases ( obese gene) in 115 Duroc and 180 hybrid pigs. The alleles were characterised with these fragments: GH- HaeII: allele - = 506bp; allele + = 333, 173bp; GH-MspI: allele - = 284, 222bp;allele + = 222, 147, 137bp; Lep: allele T = 152bp, allele C = 84, 68bp. Frequencies of genotypes of all involved genes in both populations showed a concordant distribution of genotypes and were in a genetic equilibrium according to the Hardy-Weinberg law. We found the statistically significantly different GH-HaeII and Lep gene distribution between breed Duroc and hybrid pigs.

Druh

A - Audiovizuální tvorba

CEP obor

GI - Šlechtění a plemenářství hospodářských zvířat

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

ISBN

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Místo vydání

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Název nakladatele resp. objednatele

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Verze

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