Somatic embryos development from cucumber (Cucumis sativus L.) cell suspension

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A_____%2F01%3A21100014" target="_blank" >RIV/62156489:_____/01:21100014 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Somatic embryos development from cucumber (Cucumis sativus L.) cell suspension

Popis výsledku v původním jazyce

Malepszy and Solarek were the first who report somatic embryogenesis from cucumber cell suspension in 1986. The main Iimitation of cucumber embryogenesis is a high occurrence of abnormal embryos combined with a Iow ability and Branchard, 1993). For thiswork, we report on the induction of somatic embryos in cucumber hypocotyl suspension. The endogenous ABA Ievel in the cells are also discussed. One-week-old plants, derived from the surface sterilized seeds of cucumber (Cucumis sativus L., cv. Nora) grown on a solidified medium (MS, sucrose 5 9 r'), were used. Embryogenic cell suspension cultures from hypocotyl segments of cucumber have been initiated by 7-day exposure to MS medium supplemented with 3% sucrose, 2.2 mg r' 2,4-dichlorophenoxyacetic acid,and 100 mg r' casein hydrolyzate. The suspension cultures were maintained in the same liquid medium with 1.1 mg r1 2,4-D only on a horizontal shaker at 28°C, and 16/8 h photoperiod.

Název v anglickém jazyce

Somatic embryos development from cucumber (Cucumis sativus L.) cell suspension

Popis výsledku anglicky

Malepszy and Solarek were the first who report somatic embryogenesis from cucumber cell suspension in 1986. The main Iimitation of cucumber embryogenesis is a high occurrence of abnormal embryos combined with a Iow ability and Branchard, 1993). For thiswork, we report on the induction of somatic embryos in cucumber hypocotyl suspension. The endogenous ABA Ievel in the cells are also discussed. One-week-old plants, derived from the surface sterilized seeds of cucumber (Cucumis sativus L., cv. Nora) grown on a solidified medium (MS, sucrose 5 9 r'), were used. Embryogenic cell suspension cultures from hypocotyl segments of cucumber have been initiated by 7-day exposure to MS medium supplemented with 3% sucrose, 2.2 mg r' 2,4-dichlorophenoxyacetic acid,and 100 mg r' casein hydrolyzate. The suspension cultures were maintained in the same liquid medium with 1.1 mg r1 2,4-D only on a horizontal shaker at 28°C, and 16/8 h photoperiod.

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GV206%2F96%2FK188" target="_blank" >GV206/96/K188: Cytokininy a auxiny v regulaci vývoje rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Xth International Conference on Plan Embryology

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

100-100

Název nakladatele

Neuveden

Místo vydání

Nitra, Slovak Republic

Místo konání akce

Nitra, Slovak Republic

Datum konání akce

1. 7. 2001

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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