Effect of pre-treatment and division of Norway spruce zygotic embryo on uptake of 14C-2,4-D and induction of somatic embryogenesis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A_____%2F01%3A21100015" target="_blank" >RIV/62156489:_____/01:21100015 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of pre-treatment and division of Norway spruce zygotic embryo on uptake of 14C-2,4-D and induction of somatic embryogenesis

Popis výsledku v původním jazyce

The main task of this work was to study reiations between uptake of 2,4-dichiorophenoxy acetic acid (2,4-D), an important tooi of sornatic embryogenesis induction, zygotic embryo pre-treatment and injury, that were found as increasing the subsequent induction of embryogenic cultures. The seeds were pre-treated in dark at 4 C under sterile distilied water for 0, 3, 5 and 7 days. The uptake of 14C-2, 4-D was significantly higher after 7 days treatment. The way of injury (division) ofzygotic embryos also modified uptake of 14C-2, 4-D to zygotic embryo and its parts. Results showed that the fastest shift of 14C-2,4-D into radicles (both intact and separated) was aligned with nearly none embryogenic cultures induction. On the contrary the Iowest uptake of 2,4-D into cotyledonary part was aligned with the highest number of achieved cultures. Hypocotyls showed intermediate results in both parameters.

Název v anglickém jazyce

Effect of pre-treatment and division of Norway spruce zygotic embryo on uptake of 14C-2,4-D and induction of somatic embryogenesis

Popis výsledku anglicky

The main task of this work was to study reiations between uptake of 2,4-dichiorophenoxy acetic acid (2,4-D), an important tooi of sornatic embryogenesis induction, zygotic embryo pre-treatment and injury, that were found as increasing the subsequent induction of embryogenic cultures. The seeds were pre-treated in dark at 4 C under sterile distilied water for 0, 3, 5 and 7 days. The uptake of 14C-2, 4-D was significantly higher after 7 days treatment. The way of injury (division) ofzygotic embryos also modified uptake of 14C-2, 4-D to zygotic embryo and its parts. Results showed that the fastest shift of 14C-2,4-D into radicles (both intact and separated) was aligned with nearly none embryogenic cultures induction. On the contrary the Iowest uptake of 2,4-D into cotyledonary part was aligned with the highest number of achieved cultures. Hypocotyls showed intermediate results in both parameters.

Druh

D - Stať ve sborníku

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/GV522%2F96%2FK186" target="_blank" >GV522/96/K186: Zachování a reprodukce genových zdrojů hlavních druhů jehličnatých a listnatých lesních stromů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Xth International Conference on Plan Embryology

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

114-114

Název nakladatele

Neuveden

Místo vydání

Nitra, Slovak Republic

Místo konání akce

Nitra, Slovak Republic

Datum konání akce

1. 7. 2001

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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