A new HpaII PCR-RFLP within the porcine receptor (PRLR) gene and study of its effect on litter size and number of teats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A_____%2F02%3A23300003" target="_blank" >RIV/62156489:_____/02:23300003 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A new HpaII PCR-RFLP within the porcine receptor (PRLR) gene and study of its effect on litter size and number of teats

Popis výsledku v původním jazyce

DNA polymorphism of the porcine prolactin receptor gene (PRLR) was investigated and used to study its effect on litter size and number of teats in pigs. By means of PRLR gene sequence homology in pig, human and other species, primers were designed for PCamplification within 5´ unknown (to date) part of the prolactin receptor gene in pigs. In this part of the gene, a new polymorphism with HpaII restriction endonuclease was detected. AluI polymorphism described before and our new HpaII polymorphism were used to study the associations with reproduction traits. Statistical analysis of 1020 litters showed that AluI locus was associated with litter size mainly in Landrace and affected the first parities, while HpaII locus of the gene was associated with thesame traits in Landrace and Large White and mainly affected numbers of weaned of pigs. The magnitude of the effect varied by population with the effects exceeding 2 pigs in Landrace line and 1 pig per litter in Large White populations.

Název v anglickém jazyce

A new HpaII PCR-RFLP within the porcine receptor (PRLR) gene and study of its effect on litter size and number of teats

Popis výsledku anglicky

DNA polymorphism of the porcine prolactin receptor gene (PRLR) was investigated and used to study its effect on litter size and number of teats in pigs. By means of PRLR gene sequence homology in pig, human and other species, primers were designed for PCamplification within 5´ unknown (to date) part of the prolactin receptor gene in pigs. In this part of the gene, a new polymorphism with HpaII restriction endonuclease was detected. AluI polymorphism described before and our new HpaII polymorphism were used to study the associations with reproduction traits. Statistical analysis of 1020 litters showed that AluI locus was associated with litter size mainly in Landrace and affected the first parities, while HpaII locus of the gene was associated with thesame traits in Landrace and Large White and mainly affected numbers of weaned of pigs. The magnitude of the effect varied by population with the effects exceeding 2 pigs in Landrace line and 1 pig per litter in Large White populations.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA523%2F99%2F0842" target="_blank" >GA523/99/0842: Detailní mapování QTL u prasat pomocí mikrosatelitů a kandidátních genů vybraných na základě komparativních map</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Animal Breeding and Genetics

ISSN

0931-2668

e-ISSN

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Svazek periodika

119

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GE - Gruzie

Počet stran výsledku

7

Strana od-do

57-63

Kód UT WoS článku

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EID výsledku v databázi Scopus

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