Differentiation of individual clones of long-term-cultivated somatic embryos of Norway spruce using DNA markers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A_____%2F03%3A21100009" target="_blank" >RIV/62156489:_____/03:21100009 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Differentiation of individual clones of long-term-cultivated somatic embryos of Norway spruce using DNA markers

Popis výsledku v původním jazyce

Rapidly developing methods based on molecular markers considerably contribute to the differentiation, characterisation and testing of individuals and their progeny. In our laboratory we mostly use RAPD for the differentiation of the individual clones oflong-term-cultivated somatic spruce embryos; lately we have introduced also the AFLP method. The SCAR method is used to eliminate the lower reproducibility of RAPD. For the characterisation of selected clones of somatic spruce embryos, cultivated at ourdepartment for a long time, our own clone-specific SCAR markers derived from polymorphous RAPD products were used. The RAPD fragments of DNA were cloned into plasmid vectors, sequenced and specific primers were proposed. Using PCR with these primers we are able to identify effectively the individual clones in our collection.

Název v anglickém jazyce

Differentiation of individual clones of long-term-cultivated somatic embryos of Norway spruce using DNA markers

Popis výsledku anglicky

Rapidly developing methods based on molecular markers considerably contribute to the differentiation, characterisation and testing of individuals and their progeny. In our laboratory we mostly use RAPD for the differentiation of the individual clones oflong-term-cultivated somatic spruce embryos; lately we have introduced also the AFLP method. The SCAR method is used to eliminate the lower reproducibility of RAPD. For the characterisation of selected clones of somatic spruce embryos, cultivated at ourdepartment for a long time, our own clone-specific SCAR markers derived from polymorphous RAPD products were used. The RAPD fragments of DNA were cloned into plasmid vectors, sequenced and specific primers were proposed. Using PCR with these primers we are able to identify effectively the individual clones in our collection.

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GP521%2F03%2FP148" target="_blank" >GP521/03/P148: Studium genetické variability a stability dlouhodobých kultur somatických embryí smrku ztepilého</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

7th International congress of plant molecular biology

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

412-412

Název nakladatele

ISPMB

Místo vydání

Barcelona, Spain

Místo konání akce

Barcelona, Spain

Datum konání akce

23. 6. 2003

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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