Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A_____%2F03%3A21100018" target="_blank" >RIV/62156489:_____/03:21100018 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker

Popis výsledku v původním jazyce

In 1997 and 1998 we used samples of harvested grain to verify the possibility of distinguishing 14 winter wheat genotypes and six triticale genotypes and detecting the impurity on the basis of the detection of polymorphism of prolamin kernel proteins using the methods of the PAGE ISTA. On the basis of the identity index two sister prolamin Iines with different percentage of participation, which was based on the weather conditions of the year of harvest, were discovered in seven wheat genotypes (Astella,Brea, Hana, Ilona, Siria, Sofia and Šárka) and two triticale genotypes (Tomádo and KM 779). A foreign genotype was detected in the Hana and Astella varieties. The identity index of the impurity to the Astella and Hana variety (i.e. ii = 0. 28 and ii = 0.20, respectively) was considerably Iower. In an unknown genotype (impurity) we detected the gliadin block Gld lB3, which is the genetic marker of rye translocation TIBL.IRS, the Sr31 gene of resistance to black rust.

Název v anglickém jazyce

Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker

Popis výsledku anglicky

In 1997 and 1998 we used samples of harvested grain to verify the possibility of distinguishing 14 winter wheat genotypes and six triticale genotypes and detecting the impurity on the basis of the detection of polymorphism of prolamin kernel proteins using the methods of the PAGE ISTA. On the basis of the identity index two sister prolamin Iines with different percentage of participation, which was based on the weather conditions of the year of harvest, were discovered in seven wheat genotypes (Astella,Brea, Hana, Ilona, Siria, Sofia and Šárka) and two triticale genotypes (Tomádo and KM 779). A foreign genotype was detected in the Hana and Astella varieties. The identity index of the impurity to the Astella and Hana variety (i.e. ii = 0. 28 and ii = 0.20, respectively) was considerably Iower. In an unknown genotype (impurity) we detected the gliadin block Gld lB3, which is the genetic marker of rye translocation TIBL.IRS, the Sr31 gene of resistance to black rust.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GC - Pěstování rostlin, osevní postupy

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant, Soil and environment

ISSN

1214-1178

e-ISSN

—

Svazek periodika

49

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

95-98

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—