Identification of grapevine varieties by molecular genetic method RAPD

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A_____%2F03%3A57100002" target="_blank" >RIV/62156489:_____/03:57100002 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of grapevine varieties by molecular genetic method RAPD

Popis výsledku v původním jazyce

Grapevine cultivars registered in the Czech Republic were a subject of RAPD analysis. DNA was isolated from leaflets by using commercial kit from the firm Qiagen. Nine varieties which are supposed to be genetically different and one hundred and twenty primers made by Operon, were used for screening of RAPD method. Sixteen primers producing reproducible and polymorphic bands were used for amplification of all registered varieties.A dendrogram of genetic relationship and a methodology of identification were created for each variety. It was possible to positively identify twenty six of the fifty one varieties registered in the Czech Republic just by using present bands. Nine varieties were identified only on the basis of present and absent bands. Some varieties, which are very close relative, formed small groups. It was possible to identify these varieties only as whole clusters. Two varieties were not identifiable at all. The array of the varieties will be furthermore tested by SSR metho

Název v anglickém jazyce

Identification of grapevine varieties by molecular genetic method RAPD

Popis výsledku anglicky

Grapevine cultivars registered in the Czech Republic were a subject of RAPD analysis. DNA was isolated from leaflets by using commercial kit from the firm Qiagen. Nine varieties which are supposed to be genetically different and one hundred and twenty primers made by Operon, were used for screening of RAPD method. Sixteen primers producing reproducible and polymorphic bands were used for amplification of all registered varieties.A dendrogram of genetic relationship and a methodology of identification were created for each variety. It was possible to positively identify twenty six of the fifty one varieties registered in the Czech Republic just by using present bands. Nine varieties were identified only on the basis of present and absent bands. Some varieties, which are very close relative, formed small groups. It was possible to identify these varieties only as whole clusters. Two varieties were not identifiable at all. The array of the varieties will be furthermore tested by SSR metho

Druh

D - Stať ve sborníku

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QD1153" target="_blank" >QD1153: Optimalizace technologických postupů při integrovaném pěstování listové zeleniny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Veda mladých 2003, CD - ROM

ISBN

80-8069-264-5

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

"xx"-"xy"

Název nakladatele

Slovenská poznohospodárská univezita v Nitre

Místo vydání

Nitra, SR

Místo konání akce

Račková dolina, SK

Datum konání akce

6. 11. 2003

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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