Conception rate after sex determination and cryopreservation of D7 bovine embryos

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F10%3A00003003" target="_blank" >RIV/62157124:16170/10:00003003 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Conception rate after sex determination and cryopreservation of D7 bovine embryos

Popis výsledku v původním jazyce

We evaluated conception rate after sex determination and subsequent freezing of D 7 bovine embryos and compared it with transfer of fresh female embryos. Fresh embryos were blade biopsied (n=172) and then transferred. High quality embryos (n=112) were biopsied by cell aspiration and cryopreserved. Sex determination (PCR using primers for the Y-chromosome determinant) was successfully completed in 91.3% of fresh (44% female) and in 87.5% of freeze-thawed embryos (45.9% female). The pregnancy rate was 56.5% after fresh transfer of sexed (female) embryos whereas intact embryos (control 1) implanted in 61.9% recipients. 95.6% (43/45) of embryos survived biopsy and cryopreservation. Pregnancy rate after transfer of freeze-thawed sexed (female) embryos was 48.8%. A similar conception rate was found in intact embryos (50.7%, control 2). The results demonstrate that these techniques allow the rapid commercial exchange of genetic resources on the basis of fresh or frozen sex-desired embryos in

Název v anglickém jazyce

Conception rate after sex determination and cryopreservation of D7 bovine embryos

Popis výsledku anglicky

We evaluated conception rate after sex determination and subsequent freezing of D 7 bovine embryos and compared it with transfer of fresh female embryos. Fresh embryos were blade biopsied (n=172) and then transferred. High quality embryos (n=112) were biopsied by cell aspiration and cryopreserved. Sex determination (PCR using primers for the Y-chromosome determinant) was successfully completed in 91.3% of fresh (44% female) and in 87.5% of freeze-thawed embryos (45.9% female). The pregnancy rate was 56.5% after fresh transfer of sexed (female) embryos whereas intact embryos (control 1) implanted in 61.9% recipients. 95.6% (43/45) of embryos survived biopsy and cryopreservation. Pregnancy rate after transfer of freeze-thawed sexed (female) embryos was 48.8%. A similar conception rate was found in intact embryos (50.7%, control 2). The results demonstrate that these techniques allow the rapid commercial exchange of genetic resources on the basis of fresh or frozen sex-desired embryos in

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární medicína

ISSN

0375-8427

e-ISSN

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Svazek periodika

55

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000275478200002

EID výsledku v databázi Scopus

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