Expression and serological reactivity of Nsp7 protein of PRRS genotype I virus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F12%3A43871160" target="_blank" >RIV/62157124:16170/12:43871160 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16810/12:43871688

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.rvsc.2012.06.007" target="_blank" >http://dx.doi.org/10.1016/j.rvsc.2012.06.007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.rvsc.2012.06.007" target="_blank" >10.1016/j.rvsc.2012.06.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Expression and serological reactivity of Nsp7 protein of PRRS genotype I virus

Popis výsledku v původním jazyce

The aim of this work was to express recombinant nonstructural Nsp7 protein of European genotype of porcine reproductive and respiratory syndrome virus and to evaluate its diagnostic sensitivity and specificity in serological diagnostics of the disease. The gene coding for Nsp7 protein was expressed in Escherichia coli cells and purified by IMAC. Serological reactivity of purified protein was assessed on a panel of swine sera in indirect ELISA test. Serum samples originated from PRRS positive farms, herds free of PRRS infection and PRRS free herds vaccinated with an inactivated vaccine. Nsp7 antigen proved to be suitable for serological detection of PRRS specific antibodies, showing diagnostic sensitivity of 82.2% and specificity of 97.6% when comparedwith IDEXX ELISA test. The nonstructural protein proved to be suitable for use as an antigen for the differentiation of post-infection and post-vaccination antibodies in pigs vaccinated with the inactivated vaccine. But the low overall an

Název v anglickém jazyce

Expression and serological reactivity of Nsp7 protein of PRRS genotype I virus

Popis výsledku anglicky

The aim of this work was to express recombinant nonstructural Nsp7 protein of European genotype of porcine reproductive and respiratory syndrome virus and to evaluate its diagnostic sensitivity and specificity in serological diagnostics of the disease. The gene coding for Nsp7 protein was expressed in Escherichia coli cells and purified by IMAC. Serological reactivity of purified protein was assessed on a panel of swine sera in indirect ELISA test. Serum samples originated from PRRS positive farms, herds free of PRRS infection and PRRS free herds vaccinated with an inactivated vaccine. Nsp7 antigen proved to be suitable for serological detection of PRRS specific antibodies, showing diagnostic sensitivity of 82.2% and specificity of 97.6% when comparedwith IDEXX ELISA test. The nonstructural protein proved to be suitable for use as an antigen for the differentiation of post-infection and post-vaccination antibodies in pigs vaccinated with the inactivated vaccine. But the low overall an

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Research in Veterinary Science

ISSN

0034-5288

e-ISSN

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Svazek periodika

93

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

1537-1542

Kód UT WoS článku

000311185900077

EID výsledku v databázi Scopus

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