Questing for the identity of Hepatozoon in foxes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F14%3A43873296" target="_blank" >RIV/62157124:16170/14:43873296 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Questing for the identity of Hepatozoon in foxes

Popis výsledku v původním jazyce

Apicomplexan parasites of genus Hepatozoon invade the blood cells of many mammalian species, being transmitted by range of arthropods. In domestic carnivores, three species of Hepatozoon were described to date: H. canis and H. americanum in dogs and H. felis in cats. The classification of Hepatozoon in wild carnivores is still not complete due to lack of field and experimental data as well as phylogenetic studies. The aim of this study is to carry out a survey on the prevalence and diversity of Hepatozoon sp. in red foxes Vulpes vulpes. Samples of tissues were collected from dead foxes in 11 counties of Romania; 91 samples of liver tissue were examined in total. DNA extraction was performed with commercial kit according to the manufacture's protocol. Hepatozoon sp. DNA was detected by PCR using primers amplifying 400-600 bp long part of 18S rRNA gene. These primers are commonly used for diagnostic purposes in dogs and in some studies also for detection of Hepatozoon sp. in wild carnivo

Název v anglickém jazyce

Questing for the identity of Hepatozoon in foxes

Popis výsledku anglicky

Apicomplexan parasites of genus Hepatozoon invade the blood cells of many mammalian species, being transmitted by range of arthropods. In domestic carnivores, three species of Hepatozoon were described to date: H. canis and H. americanum in dogs and H. felis in cats. The classification of Hepatozoon in wild carnivores is still not complete due to lack of field and experimental data as well as phylogenetic studies. The aim of this study is to carry out a survey on the prevalence and diversity of Hepatozoon sp. in red foxes Vulpes vulpes. Samples of tissues were collected from dead foxes in 11 counties of Romania; 91 samples of liver tissue were examined in total. DNA extraction was performed with commercial kit according to the manufacture's protocol. Hepatozoon sp. DNA was detected by PCR using primers amplifying 400-600 bp long part of 18S rRNA gene. These primers are commonly used for diagnostic purposes in dogs and in some studies also for detection of Hepatozoon sp. in wild carnivo

Druh

O - Ostatní výsledky

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů