The effect of azotemia on erythrocyte osmotic fragility in dogs with chronic and end-stage renal failure.

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of azotemia on erythrocyte osmotic fragility in dogs with chronic and end-stage renal failure.

Popis výsledku v původním jazyce

Objective: Dogs with chronic renal failure (CRF) may suffer from extreme azotemia. Urea enters erythrocytes (RBC) through its membrane. Once there, it destabilize proteins and change the set point of volume of RBC to lower values. The RBC with smaller MCV is more resistant to hypotonic environment. Previous studies on osmotic fragility revealed contrarily results. The aim of this study was to find an effect of azotaemia caused by chronic renal failure on RBC in dogs. Methods: Thirteen dogs with CRF andeighteen healthy dogs were enrolled in this study. Twenty-five microliters of heparin blood was mixed with 2,5ml of NaCl solution with decreasing concentration 0,9% - 0,1%. After incubation of 30 minutes and centrifugation the absorbance of the supernatant was measured by ELISA reader at wavelength of 540 nm. The cumulative curve was made. Median, minimal and maximal erythrocyte osmotic fragility (OF) was examined from the curve as a concentration of NaCl at which 50%, 5% and 90% of the

Název v anglickém jazyce

The effect of azotemia on erythrocyte osmotic fragility in dogs with chronic and end-stage renal failure.

Popis výsledku anglicky

Objective: Dogs with chronic renal failure (CRF) may suffer from extreme azotemia. Urea enters erythrocytes (RBC) through its membrane. Once there, it destabilize proteins and change the set point of volume of RBC to lower values. The RBC with smaller MCV is more resistant to hypotonic environment. Previous studies on osmotic fragility revealed contrarily results. The aim of this study was to find an effect of azotaemia caused by chronic renal failure on RBC in dogs. Methods: Thirteen dogs with CRF andeighteen healthy dogs were enrolled in this study. Twenty-five microliters of heparin blood was mixed with 2,5ml of NaCl solution with decreasing concentration 0,9% - 0,1%. After incubation of 30 minutes and centrifugation the absorbance of the supernatant was measured by ELISA reader at wavelength of 540 nm. The cumulative curve was made. Median, minimal and maximal erythrocyte osmotic fragility (OF) was examined from the curve as a concentration of NaCl at which 50%, 5% and 90% of the

Druh

O - Ostatní výsledky

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů