Atypical myopathy - metabolite profiling of equine serum and urine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F15%3A43874004" target="_blank" >RIV/62157124:16170/15:43874004 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Atypical myopathy - metabolite profiling of equine serum and urine

Popis výsledku v původním jazyce

Acquired equine multiple acyl-CoA dehydrogenase deficiency also known as atypical myopathy (AM), is a highly fatal muscle disease of grazing horses which in at least 75 % of cases leads to death. It is probably caused by ingestion of Acer pseudoplatanusseeds containing hypoglycin A, whose active metabolite is responsible for an inhibition of some dehydrogenases (short and medium chain acyl-CoA dehydrogenases, isovaleryl-CoA dehydrogenase etc.). The aim of this work was to perform whole metabolomic analysis in order to find other metabolic pathways which may be affected. Nine serum and six urine samples from horses with AM and twelve control samples were collected and then analyzed using high performance liquid chromatography (Ultimate 3000, Dionex) with aminopropyl column (Luna 3 ?m NH2, 2 x 100 mm, Phenomenex) coupled to tandem mass spectrometry (QTRAP 5500, AB Sciex). The results of equine serum and urine analyses show obvious separation of group with AM and control group. The diffe

Název v anglickém jazyce

Atypical myopathy - metabolite profiling of equine serum and urine

Popis výsledku anglicky

Acquired equine multiple acyl-CoA dehydrogenase deficiency also known as atypical myopathy (AM), is a highly fatal muscle disease of grazing horses which in at least 75 % of cases leads to death. It is probably caused by ingestion of Acer pseudoplatanusseeds containing hypoglycin A, whose active metabolite is responsible for an inhibition of some dehydrogenases (short and medium chain acyl-CoA dehydrogenases, isovaleryl-CoA dehydrogenase etc.). The aim of this work was to perform whole metabolomic analysis in order to find other metabolic pathways which may be affected. Nine serum and six urine samples from horses with AM and twelve control samples were collected and then analyzed using high performance liquid chromatography (Ultimate 3000, Dionex) with aminopropyl column (Luna 3 ?m NH2, 2 x 100 mm, Phenomenex) coupled to tandem mass spectrometry (QTRAP 5500, AB Sciex). The results of equine serum and urine analyses show obvious separation of group with AM and control group. The diffe

Druh

O - Ostatní výsledky

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů