Evaluation of serum and ascitic fluid proteomes in dogs with dilated cardiomyopathy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F16%3A43874105" target="_blank" >RIV/62157124:16170/16:43874105 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.9775/kvfd.2015.14429" target="_blank" >http://dx.doi.org/10.9775/kvfd.2015.14429</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.9775/kvfd.2015.14429" target="_blank" >10.9775/kvfd.2015.14429</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of serum and ascitic fluid proteomes in dogs with dilated cardiomyopathy

Popis výsledku v původním jazyce

The aim of the study was to investigate serum global proteomes in dogs with overt dilated cardiomyopathy (DCM) and to evaluate protein expression in serum with that in ascitic fluid. Eight healthy dogs (control group) and 8 dogs with DCM were included in the study. DCM was diagnosed based on echocardiographic evidence including increased left ventricular dimension at diastole and systole, increased E point to septal separation, and decreased fractional shortening. Serum and ascitic fluid samples were analyzed for proteomes using a label-free LC-MS/MS method. Proteome analyses revealed significantly different expressions of eight proteins in all samples. Expressions in serum of apolipoprotein (Apo) A1, Ig heavy chain V, superoxide dismutase and plasminogen were higher (P<0.001), while expressions of clusterin, hemoglobin subunit ß, Apo-CII, and ? 2 glycoprotein I (ß2GPI) were lower (p<0.001) in dogs with DCM than in control dogs. In addition, Apo-A1, clusterin, hemoglobin subunit ß, Ig heavy chain V, plasminogen and ß2GPI were down-regulated whereas Apo-CII and superoxide dismutase were up-regulated in ascitic fluid compared with serum in dogs with DCM. Data obtained in the present study suggest that serum and/or ascitic fluid proteomes may explain some of the pathophysiological mechanisms involved in the progression of DCM Dilate Kardiyomiyopatili Köpeklerde Serum ve Asites S?v?s? Proteomlar?n?n Araşt?r?lmas?. Available from: https://www.researchgate.net/publication/283789562_Dilate_Kardiyomiyopatili_Kopeklerde_Serum_ve_Asites_Sivisi_Proteomlarinin_Arastirilmasi [accessed Jan 8, 2016].

Název v anglickém jazyce

Evaluation of serum and ascitic fluid proteomes in dogs with dilated cardiomyopathy

Popis výsledku anglicky

The aim of the study was to investigate serum global proteomes in dogs with overt dilated cardiomyopathy (DCM) and to evaluate protein expression in serum with that in ascitic fluid. Eight healthy dogs (control group) and 8 dogs with DCM were included in the study. DCM was diagnosed based on echocardiographic evidence including increased left ventricular dimension at diastole and systole, increased E point to septal separation, and decreased fractional shortening. Serum and ascitic fluid samples were analyzed for proteomes using a label-free LC-MS/MS method. Proteome analyses revealed significantly different expressions of eight proteins in all samples. Expressions in serum of apolipoprotein (Apo) A1, Ig heavy chain V, superoxide dismutase and plasminogen were higher (P<0.001), while expressions of clusterin, hemoglobin subunit ß, Apo-CII, and ? 2 glycoprotein I (ß2GPI) were lower (p<0.001) in dogs with DCM than in control dogs. In addition, Apo-A1, clusterin, hemoglobin subunit ß, Ig heavy chain V, plasminogen and ß2GPI were down-regulated whereas Apo-CII and superoxide dismutase were up-regulated in ascitic fluid compared with serum in dogs with DCM. Data obtained in the present study suggest that serum and/or ascitic fluid proteomes may explain some of the pathophysiological mechanisms involved in the progression of DCM Dilate Kardiyomiyopatili Köpeklerde Serum ve Asites S?v?s? Proteomlar?n?n Araşt?r?lmas?. Available from: https://www.researchgate.net/publication/283789562_Dilate_Kardiyomiyopatili_Kopeklerde_Serum_ve_Asites_Sivisi_Proteomlarinin_Arastirilmasi [accessed Jan 8, 2016].

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Kafkas Universitesi Veteriner Fakultesi Dergisi

ISSN

1300-6045

e-ISSN

—

Svazek periodika

22

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

TR - Turecká republika

Počet stran výsledku

7

Strana od-do

273-279

Kód UT WoS článku

000374311900017

EID výsledku v databázi Scopus

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