Filarioid helminths in mosquitoes from the Danube Delta/Romania and the analysis of these vectors for potential vector competence

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F17%3A43875355" target="_blank" >RIV/62157124:16170/17:43875355 - isvavai.cz</a>

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RIV/62157124:16810/17:43875355

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Filarioid helminths in mosquitoes from the Danube Delta/Romania and the analysis of these vectors for potential vector competence

Popis výsledku v původním jazyce

In the past decades both Dirofilaria immitis and D. repens have spread from historically endemic areas to central and eastern European countries. Several studies have shown that Dirofilaria species are present in the southern and south-eastern areas of Romania [1]. However, information about the vectors in the Danube Delta and their vector competence is lacking. In July 2015 more than 5,000 mosquitoes were collected in the Danube Delta in Romania at various locations (including mosquito traps next to a dog infected with both D. immits and D. repens). Mosquitoes were classified to species-level using the key after Becker et.al. [2]. In one part of the study specified mosquitoes were pooled (up to 25 individuals per day/trap/mosquito species). DNA was extracted and the samples were screened for filarioid helminths using conventional PCRs. For the second part of the study 300 specified mosquito individuals caught at the trap next to a microfilariaemic dog positive for D. immitis and D. repens were segregated into head/thorax and abdomen prior to DNA extraction. Each thorax/head and abdomen was screened for the presence of filarioid DNA separately. All positive PCR products were further analysed by sequencing. Mosquitoes were sampled within the training school of WG1 under the frame of EurNegVec COST Action TD1303. Parts of this study were funded by the ERA-Net BiodivERsA, with the national funders FWF I-1437, ANR-13-EBID-0007-01 and DFG BiodivERsA KL 2087/6-1 as part of the 2012-13 BiodivERsA call for research proposals.

Název v anglickém jazyce

Filarioid helminths in mosquitoes from the Danube Delta/Romania and the analysis of these vectors for potential vector competence

Popis výsledku anglicky

In the past decades both Dirofilaria immitis and D. repens have spread from historically endemic areas to central and eastern European countries. Several studies have shown that Dirofilaria species are present in the southern and south-eastern areas of Romania [1]. However, information about the vectors in the Danube Delta and their vector competence is lacking. In July 2015 more than 5,000 mosquitoes were collected in the Danube Delta in Romania at various locations (including mosquito traps next to a dog infected with both D. immits and D. repens). Mosquitoes were classified to species-level using the key after Becker et.al. [2]. In one part of the study specified mosquitoes were pooled (up to 25 individuals per day/trap/mosquito species). DNA was extracted and the samples were screened for filarioid helminths using conventional PCRs. For the second part of the study 300 specified mosquito individuals caught at the trap next to a microfilariaemic dog positive for D. immitis and D. repens were segregated into head/thorax and abdomen prior to DNA extraction. Each thorax/head and abdomen was screened for the presence of filarioid DNA separately. All positive PCR products were further analysed by sequencing. Mosquitoes were sampled within the training school of WG1 under the frame of EurNegVec COST Action TD1303. Parts of this study were funded by the ERA-Net BiodivERsA, with the national funders FWF I-1437, ANR-13-EBID-0007-01 and DFG BiodivERsA KL 2087/6-1 as part of the 2012-13 BiodivERsA call for research proposals.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů