Diversity and host specificity of strongylid nematodes in primates: lessons learned from a next generation sequencing approach.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F17%3A43875507" target="_blank" >RIV/62157124:16170/17:43875507 - isvavai.cz</a>
Výsledek na webu
—
DOI - Digital Object Identifier
—
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Diversity and host specificity of strongylid nematodes in primates: lessons learned from a next generation sequencing approach.
Popis výsledku v původním jazyce
Strongylid nematodes tend to occur in their vertebrate hosts in rather complex communities comprised of species of several genera, especially in larger herbivorous hosts. The close phylogenetic relationship between humans and other primates results in partial overlaps of their strongylids. The interface for infectious disease transmission has changed in recent years due to closer and more frequent contact between wild primates and people. Diagnostics of strongylid nematodes is traditionally based on the presence of eggs/larvae in fecal samples, while the taxonomy largely depends on the morphology of adult worms residing in the gut. Broader application of DNA-based techniques is complicated by the absence of reference sequences for the majority of taxa and by mixed infections. We have been studying strongylid nematode diversity and transmission between free ranging African great apes, other primates including humans co-inhabiting tropical forest habitats in model site in Central Africa, using newly developed next generation sequencing (NGS) assays at the Illumina Miseq platform, targeting nuclear and mitochondrial markers and examining ~300 fecal samples and isolated larvae. We briefly describe progress to date, and present results demonstrating the diversity of strongylid communities in lowland gorillas, agile mangabeys and humans, together with host-specificity data of detected haplotypes. In conclusion, the NGS approach provides valuable insight into strongylid host specificity and diversity and is widely applicable to studies addressing vertebrate strongyle communities and their zoonotic potential. However, traditional helminthological studies are urgently needed to expand the range of sequences from well-known nematode taxa.
Název v anglickém jazyce
Diversity and host specificity of strongylid nematodes in primates: lessons learned from a next generation sequencing approach.
Popis výsledku anglicky
Strongylid nematodes tend to occur in their vertebrate hosts in rather complex communities comprised of species of several genera, especially in larger herbivorous hosts. The close phylogenetic relationship between humans and other primates results in partial overlaps of their strongylids. The interface for infectious disease transmission has changed in recent years due to closer and more frequent contact between wild primates and people. Diagnostics of strongylid nematodes is traditionally based on the presence of eggs/larvae in fecal samples, while the taxonomy largely depends on the morphology of adult worms residing in the gut. Broader application of DNA-based techniques is complicated by the absence of reference sequences for the majority of taxa and by mixed infections. We have been studying strongylid nematode diversity and transmission between free ranging African great apes, other primates including humans co-inhabiting tropical forest habitats in model site in Central Africa, using newly developed next generation sequencing (NGS) assays at the Illumina Miseq platform, targeting nuclear and mitochondrial markers and examining ~300 fecal samples and isolated larvae. We briefly describe progress to date, and present results demonstrating the diversity of strongylid communities in lowland gorillas, agile mangabeys and humans, together with host-specificity data of detected haplotypes. In conclusion, the NGS approach provides valuable insight into strongylid host specificity and diversity and is widely applicable to studies addressing vertebrate strongyle communities and their zoonotic potential. However, traditional helminthological studies are urgently needed to expand the range of sequences from well-known nematode taxa.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
—
OECD FORD obor
40301 - Veterinary science
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů