Plasmid-mediated resistance to cephalosporins and quinolones in Escherichia coli from American crows in the USA
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F17%3A43875830" target="_blank" >RIV/62157124:16170/17:43875830 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62157124:16270/17:43875830 RIV/62157124:16810/17:43875830
Výsledek na webu
<a href="http://dx.doi.org/10.1111/1462-2920.13722" target="_blank" >http://dx.doi.org/10.1111/1462-2920.13722</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/1462-2920.13722" target="_blank" >10.1111/1462-2920.13722</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Plasmid-mediated resistance to cephalosporins and quinolones in Escherichia coli from American crows in the USA
Popis výsledku v původním jazyce
American crow (Corvus brachyrhynchos) faeces were tested for Escherichia coli with plasmid-mediated quinolone resistance (PMQR), extended-spectrum beta-lactamases (ESBL) and AmpC beta-lactamases. A total of 590 faecal samples were collected at four roosting sites in the USA and cultivated on selective media. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were performed to assess clonality. Transferability of resistance genes was studied using conjugation and transformation bioassays. In total, 78 (13%, n=590) cefotaxime-resistant isolates were obtained, of which 66 and 12 displayed AmpC and ESBL phenotypes, respectively. Fifty-four AmpC-producing isolates carried bla(CMY-2). Isolates producing ESBLs contained genes bla(CTX-M-27) (5 isolates), bla(CTX-M-15) (4), bla(CTX-M-14) (2) and bla(CTX-M-1) (1). Ninety isolates (15%, n=590) with reduced susceptibility to ciprofloxacin were obtained, among which 14 harboured PMQR genes aac(6)-Ib-cr (4 isolates), qnrB19 (3), qnrS1 (2), qnrA1 (2), qnrB2 (1), qnrB6 (1) and qnrD3 (1). High genetic diversity was revealed by PFGE and MLST. Epidemiologically important E. coli clones (e.g., ST131, ST405) were identified. Plasmids carrying bla(CMY-2) were assigned predominantly to IncA/C (8 plasmids), IncI1/ST23 (5) and IncI1/ST12 (3). The study demonstrates a widespread occurrence of E. coli with ESBL, AmpC and PMQR genes associated with clinically important multidrug-resistant clones and epidemic plasmids, in American crows.
Název v anglickém jazyce
Plasmid-mediated resistance to cephalosporins and quinolones in Escherichia coli from American crows in the USA
Popis výsledku anglicky
American crow (Corvus brachyrhynchos) faeces were tested for Escherichia coli with plasmid-mediated quinolone resistance (PMQR), extended-spectrum beta-lactamases (ESBL) and AmpC beta-lactamases. A total of 590 faecal samples were collected at four roosting sites in the USA and cultivated on selective media. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were performed to assess clonality. Transferability of resistance genes was studied using conjugation and transformation bioassays. In total, 78 (13%, n=590) cefotaxime-resistant isolates were obtained, of which 66 and 12 displayed AmpC and ESBL phenotypes, respectively. Fifty-four AmpC-producing isolates carried bla(CMY-2). Isolates producing ESBLs contained genes bla(CTX-M-27) (5 isolates), bla(CTX-M-15) (4), bla(CTX-M-14) (2) and bla(CTX-M-1) (1). Ninety isolates (15%, n=590) with reduced susceptibility to ciprofloxacin were obtained, among which 14 harboured PMQR genes aac(6)-Ib-cr (4 isolates), qnrB19 (3), qnrS1 (2), qnrA1 (2), qnrB2 (1), qnrB6 (1) and qnrD3 (1). High genetic diversity was revealed by PFGE and MLST. Epidemiologically important E. coli clones (e.g., ST131, ST405) were identified. Plasmids carrying bla(CMY-2) were assigned predominantly to IncA/C (8 plasmids), IncI1/ST23 (5) and IncI1/ST12 (3). The study demonstrates a widespread occurrence of E. coli with ESBL, AmpC and PMQR genes associated with clinically important multidrug-resistant clones and epidemic plasmids, in American crows.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Environmental Microbiology
ISSN
1462-2912
e-ISSN
—
Svazek periodika
19
Číslo periodika v rámci svazku
5
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
12
Strana od-do
2025-2036
Kód UT WoS článku
000400029800022
EID výsledku v databázi Scopus
—