Hyaluronic acid and TGF-?1 in dogs with hepatobiliary diseases

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F18%3A43876372" target="_blank" >RIV/62157124:16170/18:43876372 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16370/18:43876372

Výsledek na webu

<a href="http://dx.doi.org/10.2754/avb201887030231" target="_blank" >http://dx.doi.org/10.2754/avb201887030231</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb201887030231" target="_blank" >10.2754/avb201887030231</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Hyaluronic acid and TGF-?1 in dogs with hepatobiliary diseases

Popis výsledku v původním jazyce

The aim of the study was to assess serum hyaluronic acid (HA) and transforming growth factor beta 1 (TGF-?1) concentrations: 1) to differentiate hepatic fibrosis from other forms of liver disease, and 2) for the non-invasive staging of canine liver fibrosis. We also evaluated the association between serum HA concentration and the size of the shunt vessel as an indirect marker of decreased liver clearance in patients with single congenital vascular anomaly. Forty-one healthy client-owned dogs and forty dogs diagnosed with hepatobiliary disease were enrolled in the prospective study. Patients were divided into 4 subgroups: 1) congenital portosystemic shunts (CPSS); 2) parenchymal diseases (a. mild and moderate fibrosis, b. advanced fibrosis and cirrhosis); 3) hepatic neoplasia; 4) biliary tract disorders based on thorough clinical, ultrasound and histopathological examination. Serum HA and TGF-?1 concentrations were measured using ELISA. The HA concentration was significantly increased in patients with advanced liver fibrosis/cirrhosis (P &lt; 0.001) and CPSS (P &lt; 0.001) compared to healthy dogs. Using a cut-off HA concentration of 135.94 ng/ml, the sensitivity and specificity for diagnosis for advanced liver fibrosis/cirrhosis was 100% (95% CI, 50.6?100) and 90.8% (95% CI, 81.6?95.7), respectively. The TGF-?1 levels did not significantly differ among groups (P = 0.180). Negligible correlation was found between serum HA concentration and the size of portosystemic shunt vessel (rs = 0.07; P = 0.831). These findings suggest that serum HA concentration is a potential non-invasive biomarker for advanced liver fibrosis and/or cirrhosis in dogs. The utility of measuring serum concentration of TGF-?1 for diagnosing canine liver fibrosis was not supported.

Název v anglickém jazyce

Hyaluronic acid and TGF-?1 in dogs with hepatobiliary diseases

Popis výsledku anglicky

The aim of the study was to assess serum hyaluronic acid (HA) and transforming growth factor beta 1 (TGF-?1) concentrations: 1) to differentiate hepatic fibrosis from other forms of liver disease, and 2) for the non-invasive staging of canine liver fibrosis. We also evaluated the association between serum HA concentration and the size of the shunt vessel as an indirect marker of decreased liver clearance in patients with single congenital vascular anomaly. Forty-one healthy client-owned dogs and forty dogs diagnosed with hepatobiliary disease were enrolled in the prospective study. Patients were divided into 4 subgroups: 1) congenital portosystemic shunts (CPSS); 2) parenchymal diseases (a. mild and moderate fibrosis, b. advanced fibrosis and cirrhosis); 3) hepatic neoplasia; 4) biliary tract disorders based on thorough clinical, ultrasound and histopathological examination. Serum HA and TGF-?1 concentrations were measured using ELISA. The HA concentration was significantly increased in patients with advanced liver fibrosis/cirrhosis (P &lt; 0.001) and CPSS (P &lt; 0.001) compared to healthy dogs. Using a cut-off HA concentration of 135.94 ng/ml, the sensitivity and specificity for diagnosis for advanced liver fibrosis/cirrhosis was 100% (95% CI, 50.6?100) and 90.8% (95% CI, 81.6?95.7), respectively. The TGF-?1 levels did not significantly differ among groups (P = 0.180). Negligible correlation was found between serum HA concentration and the size of portosystemic shunt vessel (rs = 0.07; P = 0.831). These findings suggest that serum HA concentration is a potential non-invasive biomarker for advanced liver fibrosis and/or cirrhosis in dogs. The utility of measuring serum concentration of TGF-?1 for diagnosing canine liver fibrosis was not supported.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30502 - Other medical science

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

—

Svazek periodika

87

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

231-240

Kód UT WoS článku

000456052200006

EID výsledku v databázi Scopus

2-s2.0-85055247519