Clostridium difficile isolates derived from Czech horses are resistant to enrofloxacin; cluster to clades 1 and 5 and ribotype 033 predominates.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F19%3A43877333" target="_blank" >RIV/62157124:16170/19:43877333 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/19:10394349 RIV/00064203:_____/19:10394349

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1075996419300046?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1075996419300046?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.anaerobe.2019.01.005" target="_blank" >10.1016/j.anaerobe.2019.01.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Clostridium difficile isolates derived from Czech horses are resistant to enrofloxacin; cluster to clades 1 and 5 and ribotype 033 predominates.

Popis výsledku v původním jazyce

Clostridium difficile has been recovered from the faeces of several animal species as well as horses. Between April 2015 and October 2016, 213 samples of faeces from non-hospitalized (n ? 138) and hospitalized horses (n ? 75) were investigated and eighteen C. difficile isolates were cultured using an enrichment method. Sixteen C. difficile positive samples were identified from hospitalised horses (p &lt; 0.01). Molecular typing revealed seven ribotypes and sequence types (RT033/ST11 n ? 8, 44.4%; RT081/ST9 n ? 4, 22.2%; RT009/ST3 n ? 2, 11.1%; RT003/ST12 n ? 1, 5.6%; RT010/ST15 n ? 1, 5.6%; RT012/ ST54 n ? 1, 5.6%; RT039/ST26 n ? 1, 5.6%). Seven identified STs clustered to two clades (1 and 5). All C. difficile isolates were susceptible to amoxicillin, metronidazole, moxifloxacin, and vancomycin. One isolate (RT039) exhibited a high level of resistance to erythromycin and clindamycin (256 mg/L) and carried the ermB, adenine methylase gene. Five isolates were resistant to clindamycin at lower minimal inhibitory concentrations (MICs ? 8-16 mg/L) and were susceptible to erythromycin and also ermB negative. All isolates were resistant to enrofloxacin (MICs ranged between 4 and 32 mg/L). Eight isolates were resistant to tetracycline (MICs 12-32 mg/L). Of them, four isolates carried the tetM gene and four isolates the tetW gene. In addition, the tetracycline resistance determinants identified were: tetA (P) (n ? 4); tetB (P); and tetL (n ? 1 each). The presence of tetW or tetM, together with other tet-class mechanisms, lead to an increase in the MICs to tetracycline. C. difficile isolates derived from Czech horses are identical to the ribotypes identified in humans and carry acquired antimicrobial resistance genes whose dissemination from veterinary healthcare sector to humans should be monitored by the ?One health? approach.

Název v anglickém jazyce

Clostridium difficile isolates derived from Czech horses are resistant to enrofloxacin; cluster to clades 1 and 5 and ribotype 033 predominates.

Popis výsledku anglicky

Clostridium difficile has been recovered from the faeces of several animal species as well as horses. Between April 2015 and October 2016, 213 samples of faeces from non-hospitalized (n ? 138) and hospitalized horses (n ? 75) were investigated and eighteen C. difficile isolates were cultured using an enrichment method. Sixteen C. difficile positive samples were identified from hospitalised horses (p &lt; 0.01). Molecular typing revealed seven ribotypes and sequence types (RT033/ST11 n ? 8, 44.4%; RT081/ST9 n ? 4, 22.2%; RT009/ST3 n ? 2, 11.1%; RT003/ST12 n ? 1, 5.6%; RT010/ST15 n ? 1, 5.6%; RT012/ ST54 n ? 1, 5.6%; RT039/ST26 n ? 1, 5.6%). Seven identified STs clustered to two clades (1 and 5). All C. difficile isolates were susceptible to amoxicillin, metronidazole, moxifloxacin, and vancomycin. One isolate (RT039) exhibited a high level of resistance to erythromycin and clindamycin (256 mg/L) and carried the ermB, adenine methylase gene. Five isolates were resistant to clindamycin at lower minimal inhibitory concentrations (MICs ? 8-16 mg/L) and were susceptible to erythromycin and also ermB negative. All isolates were resistant to enrofloxacin (MICs ranged between 4 and 32 mg/L). Eight isolates were resistant to tetracycline (MICs 12-32 mg/L). Of them, four isolates carried the tetM gene and four isolates the tetW gene. In addition, the tetracycline resistance determinants identified were: tetA (P) (n ? 4); tetB (P); and tetL (n ? 1 each). The presence of tetW or tetM, together with other tet-class mechanisms, lead to an increase in the MICs to tetracycline. C. difficile isolates derived from Czech horses are identical to the ribotypes identified in humans and carry acquired antimicrobial resistance genes whose dissemination from veterinary healthcare sector to humans should be monitored by the ?One health? approach.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Anaerobe

ISSN

1075-9964

e-ISSN

—

Svazek periodika

56

Číslo periodika v rámci svazku

april

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

17-21

Kód UT WoS článku

000466619800003

EID výsledku v databázi Scopus

2-s2.0-85060932141