Detection of bacterial populations colonizing the genital tracts of jennies and their changes during the oestrous cycle

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F20%3A43878602" target="_blank" >RIV/62157124:16170/20:43878602 - isvavai.cz</a>

Výsledek na webu

<a href="https://actavet.vfu.cz/media/pdf/actavet_2020089020107.pdf" target="_blank" >https://actavet.vfu.cz/media/pdf/actavet_2020089020107.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb202089020107" target="_blank" >10.2754/avb202089020107</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection of bacterial populations colonizing the genital tracts of jennies and their changes during the oestrous cycle

Popis výsledku v původním jazyce

The aim of this study was to help improve the management of assisted reproduction of donkeys and to extend the existing information on the fertility of donkeys by qualitative and quantitative assessment of bacterial contamination of the jennies&apos; genital tract in connection with the perineum formation and oestrous cycle phases. Ten female donkeys were included in the study and were repeatedly rectally palpated and sonographically examined during the oestrous cycle. Samples were taken from the fossa clitoridis and around the cervix for microbiological examination. Each jenny was sampled twice, always at different stages of the cycle, both in the oestrus and in the dioestrus. In addition, samples from the endometrium were taken in oestrus for both microbiological and cytological examination. After collection, the samples were examined in a microbiological laboratory. There were 62 different species of microorganisms found belonging to 19 different bacterial genera. The presence of agents of mares&apos; infectious metritis Taylorella equigenitalis was not found in any of the samples. The outcome of our study is an initial mapping of the microbial colonization of the endometrium and the genital tract in jennies with correct formation of the perineal region. The hypothesis that the level of bacterial contamination was related to the oestrous cycle was not confirmed. The practical significance of the study lies mainly in the description of the composition, amount and changes of microbiota in healthy individuals during their oestrous cycle, which allows the evaluation of the risk of developing infection during the active oestrous cycle in connection with mating.

Název v anglickém jazyce

Detection of bacterial populations colonizing the genital tracts of jennies and their changes during the oestrous cycle

Popis výsledku anglicky

The aim of this study was to help improve the management of assisted reproduction of donkeys and to extend the existing information on the fertility of donkeys by qualitative and quantitative assessment of bacterial contamination of the jennies&apos; genital tract in connection with the perineum formation and oestrous cycle phases. Ten female donkeys were included in the study and were repeatedly rectally palpated and sonographically examined during the oestrous cycle. Samples were taken from the fossa clitoridis and around the cervix for microbiological examination. Each jenny was sampled twice, always at different stages of the cycle, both in the oestrus and in the dioestrus. In addition, samples from the endometrium were taken in oestrus for both microbiological and cytological examination. After collection, the samples were examined in a microbiological laboratory. There were 62 different species of microorganisms found belonging to 19 different bacterial genera. The presence of agents of mares&apos; infectious metritis Taylorella equigenitalis was not found in any of the samples. The outcome of our study is an initial mapping of the microbial colonization of the endometrium and the genital tract in jennies with correct formation of the perineal region. The hypothesis that the level of bacterial contamination was related to the oestrous cycle was not confirmed. The practical significance of the study lies mainly in the description of the composition, amount and changes of microbiota in healthy individuals during their oestrous cycle, which allows the evaluation of the risk of developing infection during the active oestrous cycle in connection with mating.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

—

Svazek periodika

89

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

107-114

Kód UT WoS článku

000548359900001

EID výsledku v databázi Scopus

—