General caspase inhibition in chondrocytes - RNASeq based comparison of OPH and FMK inhibitors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F21%3A43879383" target="_blank" >RIV/62157124:16170/21:43879383 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

General caspase inhibition in chondrocytes - RNASeq based comparison of OPH and FMK inhibitors

Popis výsledku v původním jazyce

An RNASeq based approach was used to search for a comprehensive overview of the impact of general caspase inhibition on the transcriptome profile of chondrocytes in vitro. The impact of two mostly used general caspase inhibitors, Z-VAD-FMK (FMK) and Q-VD-OPH (OPH), was examined and compared. The experiments revealed a statistically significant impact in expression of 542 genes in the FMK samples and 351 genes in the OPH samples compared to controls. Among the affected genes, 252 were upregulated and 290 were downregulated in the FMK-treated samples, whereas, expression of 163 was increased and expression of 188 genes decreased in the OPH-treated chondrocytes. Many of the affected genes are associated with pathological conditions, such as osteoarthritis and rheumatoid arthritis. The results support the importance of caspases in cartilage homeostasis and development of diseases and points to novel regulatory mechanisms.

Název v anglickém jazyce

General caspase inhibition in chondrocytes - RNASeq based comparison of OPH and FMK inhibitors

Popis výsledku anglicky

An RNASeq based approach was used to search for a comprehensive overview of the impact of general caspase inhibition on the transcriptome profile of chondrocytes in vitro. The impact of two mostly used general caspase inhibitors, Z-VAD-FMK (FMK) and Q-VD-OPH (OPH), was examined and compared. The experiments revealed a statistically significant impact in expression of 542 genes in the FMK samples and 351 genes in the OPH samples compared to controls. Among the affected genes, 252 were upregulated and 290 were downregulated in the FMK-treated samples, whereas, expression of 163 was increased and expression of 188 genes decreased in the OPH-treated chondrocytes. Many of the affected genes are associated with pathological conditions, such as osteoarthritis and rheumatoid arthritis. The results support the importance of caspases in cartilage homeostasis and development of diseases and points to novel regulatory mechanisms.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/GA19-12023S" target="_blank" >GA19-12023S: Nové fyziologické funkce pro-apoptotických cysteinových proteáz v endochondrální osifikaci</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů