Caspase-1 inhibition affects Cd36 expression and Rankl/Opg ratio in differentiating chondrocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F21%3A43879384" target="_blank" >RIV/62157124:16170/21:43879384 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Caspase-1 inhibition affects Cd36 expression and Rankl/Opg ratio in differentiating chondrocytes

Popis výsledku v původním jazyce

The conference publication follows the modulation of the expression of the fatty acid translocase Cd36 by caspase-1. This caspase plays role in inflammation, but has been studied also in relation to lipid metabolism, the same as Cd36. For this reason, connections between the two molecules were sought using chondrogenic micromass cultures treated by selective caspase-1 inhibitor. In the treated samples, the gene expression of Cd36 was significantly upregulated. Also, the expression of two important bone remodelling and cartilage/bone homeostasis markers was changed: The Opg gene was upregulated, whereas Rankl was downregulated. Immunocytofluorescence confirmed these modulations on protein level. Cd36 silencing caused opposing trends in Rankl and Opg expression. The presented results demonstrate novel functions of caspase-1 in chondrocyte differentiation and pathways connected with lipid metabolism through Cd36. The detected effect of Cd36 on the Rankl/Opg ratio is important in relation to bone remodelling and metabolism as well as pathologies such as osteoarthritis.

Název v anglickém jazyce

Caspase-1 inhibition affects Cd36 expression and Rankl/Opg ratio in differentiating chondrocytes

Popis výsledku anglicky

The conference publication follows the modulation of the expression of the fatty acid translocase Cd36 by caspase-1. This caspase plays role in inflammation, but has been studied also in relation to lipid metabolism, the same as Cd36. For this reason, connections between the two molecules were sought using chondrogenic micromass cultures treated by selective caspase-1 inhibitor. In the treated samples, the gene expression of Cd36 was significantly upregulated. Also, the expression of two important bone remodelling and cartilage/bone homeostasis markers was changed: The Opg gene was upregulated, whereas Rankl was downregulated. Immunocytofluorescence confirmed these modulations on protein level. Cd36 silencing caused opposing trends in Rankl and Opg expression. The presented results demonstrate novel functions of caspase-1 in chondrocyte differentiation and pathways connected with lipid metabolism through Cd36. The detected effect of Cd36 on the Rankl/Opg ratio is important in relation to bone remodelling and metabolism as well as pathologies such as osteoarthritis.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/GA19-12023S" target="_blank" >GA19-12023S: Nové fyziologické funkce pro-apoptotických cysteinových proteáz v endochondrální osifikaci</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů