Role of primary cilia in oral tumors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F21%3A43879473" target="_blank" >RIV/62157124:16170/21:43879473 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Role of primary cilia in oral tumors

Popis výsledku v původním jazyce

The primary cilia (PC) are immobile microtubular structures localized on the surface of most human cells. These organelles are sensory mediators, relaying environmental signals into the regulation of signaling pathways. Cilia-related pathologies are suspected to be associated with many cancers. Our goal is to uncover the function of primary cilia in the pathogenesis of oral squamous cell carcinoma (OSCC) and ameloblastoma. PCs play an essential role in Hedgehog signaling, so we focused on the genes involved in this pathway. We used immunofluorescence confocal microscopy to visualize PCs in OSCC and ameloblastoma tissues. We also performed real-time PCR to assess the primary cilia and Hedgehog signaling-related gene expression in OSCC and ameloblastoma, using healthy gingiva as a control healthy tissue. There is a significant difference between PCs occurrence in both analyzed tumors. While primary cilia are mostly absent on OSCC cells, tumorous cells of ameloblastomas display abundant and elongated primary cilia. Gli1 expression was upregulated in OSCC, while Ptch1 was slightly downregulated in comparison to gingiva. In ameloblastoma, Gli1 and Ptch1 were significantly upregulated. These findings might lead to the identification of causes of aberrant ciliogenesis in OSCC and explain different cell behavior between these tumors.

Název v anglickém jazyce

Role of primary cilia in oral tumors

Popis výsledku anglicky

The primary cilia (PC) are immobile microtubular structures localized on the surface of most human cells. These organelles are sensory mediators, relaying environmental signals into the regulation of signaling pathways. Cilia-related pathologies are suspected to be associated with many cancers. Our goal is to uncover the function of primary cilia in the pathogenesis of oral squamous cell carcinoma (OSCC) and ameloblastoma. PCs play an essential role in Hedgehog signaling, so we focused on the genes involved in this pathway. We used immunofluorescence confocal microscopy to visualize PCs in OSCC and ameloblastoma tissues. We also performed real-time PCR to assess the primary cilia and Hedgehog signaling-related gene expression in OSCC and ameloblastoma, using healthy gingiva as a control healthy tissue. There is a significant difference between PCs occurrence in both analyzed tumors. While primary cilia are mostly absent on OSCC cells, tumorous cells of ameloblastomas display abundant and elongated primary cilia. Gli1 expression was upregulated in OSCC, while Ptch1 was slightly downregulated in comparison to gingiva. In ameloblastoma, Gli1 and Ptch1 were significantly upregulated. These findings might lead to the identification of causes of aberrant ciliogenesis in OSCC and explain different cell behavior between these tumors.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů