The potential and shortcomings of mitochondrial DNA analysis for cheetah conservation management

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F22%3A43879971" target="_blank" >RIV/62157124:16170/22:43879971 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16810/22:43879971

Výsledek na webu

<a href="https://assets.researchsquare.com/files/rs-2058167/v1/249691aa-8735-4532-aaa8-40271c75d2ee.pdf?c=1663363123" target="_blank" >https://assets.researchsquare.com/files/rs-2058167/v1/249691aa-8735-4532-aaa8-40271c75d2ee.pdf?c=1663363123</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10592-022-01483-1" target="_blank" >10.1007/s10592-022-01483-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The potential and shortcomings of mitochondrial DNA analysis for cheetah conservation management

Popis výsledku v původním jazyce

There are only about 7,100 adolescent and adult cheetahs (Acinonyx jubatus) remaining in the wild. With the majority occurring outside protected areas, their numbers are rapidly declining. Evidence-based conservation measures are essential for the survival of this species. Genetic data is routinely used to inform conservation strategies, e.g., by establishing conservation units (CU). A commonly used marker in conservation genetics is mitochondrial DNA (mtDNA). Here, we investigated the cheetah&apos;s phylogeography using a large-scale mtDNA data set to refine subspecies distributions and better assign individuals to CUs. Our dataset mostly consisted of historic samples to cover the cheetah&apos;s whole range as the species has been extinct in most of its former distribution. While our genetic data largely agree with geography-based subspecies assignments, several geographic regions show conflicting mtDNA signals. Our analyses support previous findings that evolutionary forces such as incomplete lineage sorting or mitochondrial capture likely confound the mitochondrial phylogeography of this species, especially in East and, to some extent, in Northeast Africa. We caution that subspecies assignments solely based on mtDNA should be treated carefully and argue for an additional standardized nuclear single nucleotide polymorphism (SNP) marker set for subspecies identification and monitoring. However, the detection of the A. j. soemmeringii specific haplogroup by a newly designed Amplification-Refractory Mutation System (ARMS) can already provide support for conservation measures.

Název v anglickém jazyce

The potential and shortcomings of mitochondrial DNA analysis for cheetah conservation management

Popis výsledku anglicky

There are only about 7,100 adolescent and adult cheetahs (Acinonyx jubatus) remaining in the wild. With the majority occurring outside protected areas, their numbers are rapidly declining. Evidence-based conservation measures are essential for the survival of this species. Genetic data is routinely used to inform conservation strategies, e.g., by establishing conservation units (CU). A commonly used marker in conservation genetics is mitochondrial DNA (mtDNA). Here, we investigated the cheetah&apos;s phylogeography using a large-scale mtDNA data set to refine subspecies distributions and better assign individuals to CUs. Our dataset mostly consisted of historic samples to cover the cheetah&apos;s whole range as the species has been extinct in most of its former distribution. While our genetic data largely agree with geography-based subspecies assignments, several geographic regions show conflicting mtDNA signals. Our analyses support previous findings that evolutionary forces such as incomplete lineage sorting or mitochondrial capture likely confound the mitochondrial phylogeography of this species, especially in East and, to some extent, in Northeast Africa. We caution that subspecies assignments solely based on mtDNA should be treated carefully and argue for an additional standardized nuclear single nucleotide polymorphism (SNP) marker set for subspecies identification and monitoring. However, the detection of the A. j. soemmeringii specific haplogroup by a newly designed Amplification-Refractory Mutation System (ARMS) can already provide support for conservation measures.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10619 - Biodiversity conservation

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Conservation genetics

ISSN

1566-0621

e-ISSN

1572-9737

Svazek periodika

neuveden

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

nestrankovano

Kód UT WoS článku

000897356700001

EID výsledku v databázi Scopus

2-s2.0-85143592273