Detection of Capocytophaga spp. in dogs - direct PCR versus standard culture procedure

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F22%3A43880024" target="_blank" >RIV/62157124:16170/22:43880024 - isvavai.cz</a>

Výsledek na webu

<a href="https://kongrescssm2022.bpp.cz/cs/sbornik-abstrakt" target="_blank" >https://kongrescssm2022.bpp.cz/cs/sbornik-abstrakt</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of Capocytophaga spp. in dogs - direct PCR versus standard culture procedure

Popis výsledku v původním jazyce

Capnocytophaga are Gram-negative commensal bacteria found in oral flora of mammals, including dogs. They are considered an opportunistic pathogen, and some species pose a risk to human health from bites. The study aims to compare the effectiveness of two diagnostic methods, PCR and standard bacteriological cultivation combined with MALDI TOF MS, in identification of Capnocytophaga species in dogs. Samples were taken in the form of a pair of swabs taken from the gums and teeth of dogs. These were stored in Amies transport medium. DNA was isolated using NucleoSpin? Tissue Kit (Roche). Isolated DNA was used as a PCR template, in order to identify the presence of the Capnocytophaga species found in dogs. The second smear was used for cultivation of the bacteria on Heart infusion agar (Difco) supplemented by gentamicin. The agar plates were than incubated in atmosphere enriched with CO2 (5-10 %) for 2-5 days at 37°C. Bacterial colonies were than identified using MALDI TOF MS. Samples from different breeds of dogs were collected and tested. Opportunely pathogenic species C. canimorsus was found in 53 % samples by direct PCR and in 26,5 % samples by culture procedure. C. cynodegmi was found in 61,5 % samples by direct PCR and in 75,9 % samples by culture procedure. Direct PCR appears to be more effective in identifying oral colonization of dogs by C. canimorsus, while both methods appear to be equally effective in identifying C. cynodegmi colonization. However, the culture procedure is necessary for the definitive typing of virulent types of C. canimorsus.

Název v anglickém jazyce

Detection of Capocytophaga spp. in dogs - direct PCR versus standard culture procedure

Popis výsledku anglicky

Capnocytophaga are Gram-negative commensal bacteria found in oral flora of mammals, including dogs. They are considered an opportunistic pathogen, and some species pose a risk to human health from bites. The study aims to compare the effectiveness of two diagnostic methods, PCR and standard bacteriological cultivation combined with MALDI TOF MS, in identification of Capnocytophaga species in dogs. Samples were taken in the form of a pair of swabs taken from the gums and teeth of dogs. These were stored in Amies transport medium. DNA was isolated using NucleoSpin? Tissue Kit (Roche). Isolated DNA was used as a PCR template, in order to identify the presence of the Capnocytophaga species found in dogs. The second smear was used for cultivation of the bacteria on Heart infusion agar (Difco) supplemented by gentamicin. The agar plates were than incubated in atmosphere enriched with CO2 (5-10 %) for 2-5 days at 37°C. Bacterial colonies were than identified using MALDI TOF MS. Samples from different breeds of dogs were collected and tested. Opportunely pathogenic species C. canimorsus was found in 53 % samples by direct PCR and in 26,5 % samples by culture procedure. C. cynodegmi was found in 61,5 % samples by direct PCR and in 75,9 % samples by culture procedure. Direct PCR appears to be more effective in identifying oral colonization of dogs by C. canimorsus, while both methods appear to be equally effective in identifying C. cynodegmi colonization. However, the culture procedure is necessary for the definitive typing of virulent types of C. canimorsus.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů