Advancing source attributing Toxoplasma gondii serology

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Advancing source attributing Toxoplasma gondii serology

Popis výsledku v původním jazyce

Environmental transmission via oocysts is considered an important route of Toxoplasma gondii infection in humans. However, differentiation of oocyst-versus tissue cyst-driven infections is still a major gap from a One Health perspective. Serology based on oocyst specific proteins may help, but has had relevant limitations. Herein we have applied a workflow for screening proteins predicted to be specific to oocyst wall (n=6) or sporozoites (n=12). Two main issues were tackled: selecting reference sera and the lack of a reference test. Accordingly, a well characterized sera panel from 24 pigs experimentally infected with T. gondii oocysts (250 P.O.) or tissue cysts (10 P.O.) from type II and type III isolates was used (six animals per group; sera samples collected weekly prior to infection till six weeks post-infection). First, proteins were screened with sera from three pigs infected with oocysts up to three weeks post-infection by Western Blot (WB). Proteins were discarded if they were not recognized or were recognized prior to infection. Two sporozoite specific proteins were selected, CCp5A and SR1. Next, the whole panel of sera was analyzed by WB. CCp5A WB results did not show relevant differences between oocysts and tissue cyst-driven infections. However, CCp5A was recognized earlier by animals infected with oocysts from a type III isolate. These preliminary results evidenced the usefulness of the workflow for protein candidates with potential for source attribution. Additional limitations that complicate an accurate interpretation of the results have been identified. Further studies are ongoing.

Název v anglickém jazyce

Advancing source attributing Toxoplasma gondii serology

Popis výsledku anglicky

Environmental transmission via oocysts is considered an important route of Toxoplasma gondii infection in humans. However, differentiation of oocyst-versus tissue cyst-driven infections is still a major gap from a One Health perspective. Serology based on oocyst specific proteins may help, but has had relevant limitations. Herein we have applied a workflow for screening proteins predicted to be specific to oocyst wall (n=6) or sporozoites (n=12). Two main issues were tackled: selecting reference sera and the lack of a reference test. Accordingly, a well characterized sera panel from 24 pigs experimentally infected with T. gondii oocysts (250 P.O.) or tissue cysts (10 P.O.) from type II and type III isolates was used (six animals per group; sera samples collected weekly prior to infection till six weeks post-infection). First, proteins were screened with sera from three pigs infected with oocysts up to three weeks post-infection by Western Blot (WB). Proteins were discarded if they were not recognized or were recognized prior to infection. Two sporozoite specific proteins were selected, CCp5A and SR1. Next, the whole panel of sera was analyzed by WB. CCp5A WB results did not show relevant differences between oocysts and tissue cyst-driven infections. However, CCp5A was recognized earlier by animals infected with oocysts from a type III isolate. These preliminary results evidenced the usefulness of the workflow for protein candidates with potential for source attribution. Additional limitations that complicate an accurate interpretation of the results have been identified. Further studies are ongoing.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů