Direct Detection of Feline Coronavirus by Three Rapid Antigen Immunochromatographic Tests and by Real-Time PCR in Cat Shelters

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F22%3A43880154" target="_blank" >RIV/62157124:16170/22:43880154 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16270/22:43880154

Výsledek na webu

<a href="https://www.mdpi.com/2306-7381/9/2/35" target="_blank" >https://www.mdpi.com/2306-7381/9/2/35</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/vetsci9020035" target="_blank" >10.3390/vetsci9020035</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Direct Detection of Feline Coronavirus by Three Rapid Antigen Immunochromatographic Tests and by Real-Time PCR in Cat Shelters

Popis výsledku v původním jazyce

The aim of this study was the direct detection of feline coronavirus by real-time PCR and by three different rapid immunochromatographic (RIM) tests detecting antigens in faecal samples of shelter cats. Based on sensitivity and specificity calculated for each of the RIM tests, the utility of RIM tests was compared. Seventy faecal samples originating from shelter cats housed in quarantine were examined. Out of 70 samples analyzed by real-time PCR, 44 (62.9%) were positive. Significantly more cats (p &lt; 0.05) tested positive than negative. Neither age nor sex of the cats played a significant role (p &gt; 0.05) in the shedding status of the virus. The sensitivity of the RIM tests was found to be at low (&lt;35%; RIM tests A and C) to satisfactory level (&gt;50%, RIM test B). The number of virus particles determined by real-time RT-PCR analysis did not significantly correlate with the results detected by any of the RIM tests (p &gt; 0.05). The results of this study indicate that the use of rapid antigen RIM tests in routine screening of FCoV shedding status in shelter cats is limited due to the occurrence of a high number of false negative results.

Název v anglickém jazyce

Direct Detection of Feline Coronavirus by Three Rapid Antigen Immunochromatographic Tests and by Real-Time PCR in Cat Shelters

Popis výsledku anglicky

The aim of this study was the direct detection of feline coronavirus by real-time PCR and by three different rapid immunochromatographic (RIM) tests detecting antigens in faecal samples of shelter cats. Based on sensitivity and specificity calculated for each of the RIM tests, the utility of RIM tests was compared. Seventy faecal samples originating from shelter cats housed in quarantine were examined. Out of 70 samples analyzed by real-time PCR, 44 (62.9%) were positive. Significantly more cats (p &lt; 0.05) tested positive than negative. Neither age nor sex of the cats played a significant role (p &gt; 0.05) in the shedding status of the virus. The sensitivity of the RIM tests was found to be at low (&lt;35%; RIM tests A and C) to satisfactory level (&gt;50%, RIM test B). The number of virus particles determined by real-time RT-PCR analysis did not significantly correlate with the results detected by any of the RIM tests (p &gt; 0.05). The results of this study indicate that the use of rapid antigen RIM tests in routine screening of FCoV shedding status in shelter cats is limited due to the occurrence of a high number of false negative results.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Sciences

ISSN

2306-7381

e-ISSN

2306-7381

Svazek periodika

9

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

nestrankovano

Kód UT WoS článku

000762017700001

EID výsledku v databázi Scopus

—