The potential for use of CaviPlasma device for elimination pathogens in water

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F22%3A43880294" target="_blank" >RIV/62157124:16170/22:43880294 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The potential for use of CaviPlasma device for elimination pathogens in water

Popis výsledku v původním jazyce

Currently, water contamination is of high importance and researchers are looking for new methods to purify water. Researchers from Brno (Czech Republic) developed a new technology for water purification using low-temperature plasma and cavitation. This device is called CaviPlasma which is now protected by a Czech patent (Rudolf et al., 2019). CaviPlasma can remove chemical residues such as oestrogens from contraceptive products and kill pathogenic microorganisms. First experiments with CaviPlasma demonstrated its exceptionally high efficacy in purifying water contaminated with cyanobacteria (Microcystis aeruginosa) (Maršálek et al., 2019). Another study demonstrated the ability of CaviPlasmatreated water to inactivate algae and cyanobacteria (Čech et al., 2020). We are considering the possibility of using this device to eliminate fish pathogens in water. We tested efficiency of CaviPlasma on the survival of bacteria (Aeromonas salmonicida, Aeromonas hydrophila and Flavobacterium psychrophilum). Thirty ml of bacterial suspension was inoculated into three l of tap water in sterile bottles and mixed well. 500 ml was used as positive control without treatment and 2,5 l was used for CaviPlasma treatment. Water with added bacteria underwent five passages through CaviPlasma device After each passage, samples of treated water were taken for futher analysis. During the first set of experiments the water flow rate was set to 1 m3 /h (ca. 17 slpm) and the separation of electrodes was approx. 16 cm. The backpressure was lowered to set the cavitation cloud spanning from the nozzle electrode to half the electrode distance. The discharge was energized using a HV generator set to approx. 65 kHz frequency at an input power of 600 W. The total treatment time of loaded batch of contaminated water was set to 65 seconds and 105 seconds. During the second set of experiments the separation of electrodes was set to 12 cm and the cavitation cloud spanned the entire inter-electrode space. At these conditions the input power was increased to 1 kW keeping the treatment times the same as previous. Samples of treated water were cultured at 17 °C and concentration of bacteria was regularly measured during the whole cultivation using plate count method. After cultivation, grown colonies were counted as colony forming units (CFU) per ml. In the first set of experiments, Aeromonas spp. survived in the treated water. The second set of experiments affected the survival of Aeromonas ssp. The bacteria were present in low quantities and gradually their quantities were decreasing. Flavobacterium psychrophilum was killed after only one passage of water in both sets. We are testing the potential of CaviPlasma to eliminate a parasitic ciliate Ichthyophthirius multifiliis (the causative agent of the white spot disease). Water with developmental stages of Ichthyophthirius multifiliis was treated with CaviPlasma. This water underwent one or 5 passages through CaviPlasma device. Positive and negative control groups were included. The experiments on the effect of CaviPlasma on Ichthyophthirius multifiliis are in progress, the results will be presented as a poster at the conference.

Název v anglickém jazyce

The potential for use of CaviPlasma device for elimination pathogens in water

Popis výsledku anglicky

Currently, water contamination is of high importance and researchers are looking for new methods to purify water. Researchers from Brno (Czech Republic) developed a new technology for water purification using low-temperature plasma and cavitation. This device is called CaviPlasma which is now protected by a Czech patent (Rudolf et al., 2019). CaviPlasma can remove chemical residues such as oestrogens from contraceptive products and kill pathogenic microorganisms. First experiments with CaviPlasma demonstrated its exceptionally high efficacy in purifying water contaminated with cyanobacteria (Microcystis aeruginosa) (Maršálek et al., 2019). Another study demonstrated the ability of CaviPlasmatreated water to inactivate algae and cyanobacteria (Čech et al., 2020). We are considering the possibility of using this device to eliminate fish pathogens in water. We tested efficiency of CaviPlasma on the survival of bacteria (Aeromonas salmonicida, Aeromonas hydrophila and Flavobacterium psychrophilum). Thirty ml of bacterial suspension was inoculated into three l of tap water in sterile bottles and mixed well. 500 ml was used as positive control without treatment and 2,5 l was used for CaviPlasma treatment. Water with added bacteria underwent five passages through CaviPlasma device After each passage, samples of treated water were taken for futher analysis. During the first set of experiments the water flow rate was set to 1 m3 /h (ca. 17 slpm) and the separation of electrodes was approx. 16 cm. The backpressure was lowered to set the cavitation cloud spanning from the nozzle electrode to half the electrode distance. The discharge was energized using a HV generator set to approx. 65 kHz frequency at an input power of 600 W. The total treatment time of loaded batch of contaminated water was set to 65 seconds and 105 seconds. During the second set of experiments the separation of electrodes was set to 12 cm and the cavitation cloud spanned the entire inter-electrode space. At these conditions the input power was increased to 1 kW keeping the treatment times the same as previous. Samples of treated water were cultured at 17 °C and concentration of bacteria was regularly measured during the whole cultivation using plate count method. After cultivation, grown colonies were counted as colony forming units (CFU) per ml. In the first set of experiments, Aeromonas spp. survived in the treated water. The second set of experiments affected the survival of Aeromonas ssp. The bacteria were present in low quantities and gradually their quantities were decreasing. Flavobacterium psychrophilum was killed after only one passage of water in both sets. We are testing the potential of CaviPlasma to eliminate a parasitic ciliate Ichthyophthirius multifiliis (the causative agent of the white spot disease). Water with developmental stages of Ichthyophthirius multifiliis was treated with CaviPlasma. This water underwent one or 5 passages through CaviPlasma device. Positive and negative control groups were included. The experiments on the effect of CaviPlasma on Ichthyophthirius multifiliis are in progress, the results will be presented as a poster at the conference.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/QK21010030" target="_blank" >QK21010030: Globalizace, moderní technologie a změna klimatu jako zdroje nových možností a ohrožení pro chovný management lososovitých ryb</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů