Lymphocyte immunophenotyping and concentration of MMP-9 in transudates and exudates in horses

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F23%3A43880609" target="_blank" >RIV/62157124:16170/23:43880609 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0165242723000995" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0165242723000995</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vetimm.2023.110645" target="_blank" >10.1016/j.vetimm.2023.110645</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Lymphocyte immunophenotyping and concentration of MMP-9 in transudates and exudates in horses

Popis výsledku v původním jazyce

This study is the first to provide information on the lymphocyte subpopulations in peritoneal effusions in horses. Peritoneal transudates (n = 12), peritoneal exudates (n = 6) and a pleural exudate (n = 1) were analyzed. The total nucleated cell count (TNCC), total protein (TP) and matrix metalloproteinase-9 (MMP-9) concentration determined by ELISA were measured and routine cytological evaluation was performed. CD3, CD4, CD8 and CD21 positive cells were detected by flow cytometry. A higher percentage of neutrophils (P &lt; 0.05) and higher MMP-9 (P &lt; 0.01) levels were found in exudates. A higher percentage of macrophages (P &lt; 0.05) and lymphocytes (P &lt; 0.01) were found in transudates. CD4 + lymphocytes were the most common lymphocyte subpopulation in all samples. CD21 + lymphocytes were the least common in all samples. A large variability in the percentage of CD21 + lymphocytes was found in exudates. The percentage of CD21 + lymphocytes positively correlated with the level of total protein (r = 0.5704, P &lt; 0.05). The correlation was even stronger in the group of exudates. The percentages of lymphocyte subpopulations did not correlate with the level of MMP-9 or with cytological findings. The level of MMP-9 positively correlated with the percentage of neutrophils (r = 0.4980, P &lt; 0.05), the level of TP (r = 0.7855, P &lt; 0.01) and TNCC (r = 0.6129, P &lt; 0.01). A significantly higher level of MMP-9 was detected in euthanized horses than in horses that survived (P &lt; 0.05). However, it was shown that the level of MMP-9 in the peritoneal fluid can change significantly in a short time. More studies on repeated abdominocentesis could contribute to elucidating the role of MMP-9 as a prognostic indicator.

Název v anglickém jazyce

Lymphocyte immunophenotyping and concentration of MMP-9 in transudates and exudates in horses

Popis výsledku anglicky

This study is the first to provide information on the lymphocyte subpopulations in peritoneal effusions in horses. Peritoneal transudates (n = 12), peritoneal exudates (n = 6) and a pleural exudate (n = 1) were analyzed. The total nucleated cell count (TNCC), total protein (TP) and matrix metalloproteinase-9 (MMP-9) concentration determined by ELISA were measured and routine cytological evaluation was performed. CD3, CD4, CD8 and CD21 positive cells were detected by flow cytometry. A higher percentage of neutrophils (P &lt; 0.05) and higher MMP-9 (P &lt; 0.01) levels were found in exudates. A higher percentage of macrophages (P &lt; 0.05) and lymphocytes (P &lt; 0.01) were found in transudates. CD4 + lymphocytes were the most common lymphocyte subpopulation in all samples. CD21 + lymphocytes were the least common in all samples. A large variability in the percentage of CD21 + lymphocytes was found in exudates. The percentage of CD21 + lymphocytes positively correlated with the level of total protein (r = 0.5704, P &lt; 0.05). The correlation was even stronger in the group of exudates. The percentages of lymphocyte subpopulations did not correlate with the level of MMP-9 or with cytological findings. The level of MMP-9 positively correlated with the percentage of neutrophils (r = 0.4980, P &lt; 0.05), the level of TP (r = 0.7855, P &lt; 0.01) and TNCC (r = 0.6129, P &lt; 0.01). A significantly higher level of MMP-9 was detected in euthanized horses than in horses that survived (P &lt; 0.05). However, it was shown that the level of MMP-9 in the peritoneal fluid can change significantly in a short time. More studies on repeated abdominocentesis could contribute to elucidating the role of MMP-9 as a prognostic indicator.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Immunology and Immunopathology

ISSN

0165-2427

e-ISSN

1873-2534

Svazek periodika

263

Číslo periodika v rámci svazku

September

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

—

Kód UT WoS článku

001064747800001

EID výsledku v databázi Scopus

2-s2.0-85169846692