Wild boar (Sus scrofa) as a possible source of Clostridioides difficile for humans

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Wild boar (Sus scrofa) as a possible source of Clostridioides difficile for humans

Popis výsledku v původním jazyce

Clostridioides difficile (C. difficile) is one of the most important causative agents of life-threatening infections of the human colon, and until recently clostridial colitis was considered as a disease of mainly nosocomial origin. However, there has been an unusually high increase in the number of community infections in recent years. The aim of our work was to verify whether the wild boars really represent a possible risk for human colonization by toxin producing strains of C. difficile. We obtained 21 C. difficile isolates from 376 diaphragm muscle samples (5%), in thirteen (62%) of them was confirmed presence of genes encoding toxin production. Most of the toxinogenic isolates (10/13) carried the tcdA and tcdB genes, only three isolates had the cdtA/cdtB genes for the binary toxin identified. The detection of toxinogenic clostridia in wild boars may represent a risk of alimentary infection for humans in case of consumption of insufficiently heat-treated boar meat. The important finding is that none of our isolates showed resistance to the antibiotics of choice in the treatment of human pseudomembranous colitis caused by toxinogenic strains of C. difficile, which are metronidazole and vancomycin.

Název v anglickém jazyce

Wild boar (Sus scrofa) as a possible source of Clostridioides difficile for humans

Popis výsledku anglicky

Clostridioides difficile (C. difficile) is one of the most important causative agents of life-threatening infections of the human colon, and until recently clostridial colitis was considered as a disease of mainly nosocomial origin. However, there has been an unusually high increase in the number of community infections in recent years. The aim of our work was to verify whether the wild boars really represent a possible risk for human colonization by toxin producing strains of C. difficile. We obtained 21 C. difficile isolates from 376 diaphragm muscle samples (5%), in thirteen (62%) of them was confirmed presence of genes encoding toxin production. Most of the toxinogenic isolates (10/13) carried the tcdA and tcdB genes, only three isolates had the cdtA/cdtB genes for the binary toxin identified. The detection of toxinogenic clostridia in wild boars may represent a risk of alimentary infection for humans in case of consumption of insufficiently heat-treated boar meat. The important finding is that none of our isolates showed resistance to the antibiotics of choice in the treatment of human pseudomembranous colitis caused by toxinogenic strains of C. difficile, which are metronidazole and vancomycin.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů