Genetic susceptibility to sarcoid in Arab horses: associations with MHC but not with NKC genomic regions

Kód výsledku v IS VaVaI

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Nalezeny alternativní kódy

RIV/62157124:16810/24:43881452

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genetic susceptibility to sarcoid in Arab horses: associations with MHC but not with NKC genomic regions

Popis výsledku v původním jazyce

Although the Major Histocompatibility Complex (MHC) has been repeatedly associated with susceptibility to equine sarcoid, a disease associated with bovine papillomavirus (BPV1) infection, the role of the MHC in the mechanisms of the disease is not fully understood (SemikGurgul, 2021). The objectives of our work were to analyze associations between polymorphic markers of the MHC genomic subregions and of the Natural Killer Cell complex (NKC) and the presence of sarcoids in Arab horses. Microsatellite loci located in the MHC class I, II and III subregions and two MHC class II genes (DRA, DQA1), along with a set of NKC microsatelllite markers, were genotyped (Horecky et al., 2018). Fourteen microsatellites of the standard parentage kit, located outside the MHC and NKC regions, were also tested. The presence of BPV1 in cutaneous swabs was confirmed in all orses. Standard chi-square and Fisher tests with Bonferroni corrections were used for association analyses. No associations with sarcoid were found for non-MHC, non-NKC microsatellite loci, MHC class I and/or class III microsatellites, or for NKC-linked microsatellite loci. Significant associations were found between the presence of sarcoids and MHC class II-linked microsatellites CZM004, COR114, TKY3324, and COR112. The expressed genes DRA and DQA1 showed no associations. The results suggest that the MHC class II region could play a role in immune responses to sarcoid. Ongoing long-read Nanopore sequencing of the region encompassing the marker COR112 should provide more information on this part of the MHC region containing no expressed genes.

Název v anglickém jazyce

Genetic susceptibility to sarcoid in Arab horses: associations with MHC but not with NKC genomic regions

Popis výsledku anglicky

Although the Major Histocompatibility Complex (MHC) has been repeatedly associated with susceptibility to equine sarcoid, a disease associated with bovine papillomavirus (BPV1) infection, the role of the MHC in the mechanisms of the disease is not fully understood (SemikGurgul, 2021). The objectives of our work were to analyze associations between polymorphic markers of the MHC genomic subregions and of the Natural Killer Cell complex (NKC) and the presence of sarcoids in Arab horses. Microsatellite loci located in the MHC class I, II and III subregions and two MHC class II genes (DRA, DQA1), along with a set of NKC microsatelllite markers, were genotyped (Horecky et al., 2018). Fourteen microsatellites of the standard parentage kit, located outside the MHC and NKC regions, were also tested. The presence of BPV1 in cutaneous swabs was confirmed in all orses. Standard chi-square and Fisher tests with Bonferroni corrections were used for association analyses. No associations with sarcoid were found for non-MHC, non-NKC microsatellite loci, MHC class I and/or class III microsatellites, or for NKC-linked microsatellite loci. Significant associations were found between the presence of sarcoids and MHC class II-linked microsatellites CZM004, COR114, TKY3324, and COR112. The expressed genes DRA and DQA1 showed no associations. The results suggest that the MHC class II region could play a role in immune responses to sarcoid. Ongoing long-read Nanopore sequencing of the region encompassing the marker COR112 should provide more information on this part of the MHC region containing no expressed genes.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů