Identification of E. coli resistant to beta-lactam antibiotics isolated from Czech diary calves

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F24%3A43881639" target="_blank" >RIV/62157124:16170/24:43881639 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of E. coli resistant to beta-lactam antibiotics isolated from Czech diary calves

Popis výsledku v původním jazyce

Antimicrobial resistance is a major threat to human and animal health. Our study focused on beta-lactam resistance in Escherichia coli from calves in Czech farms. In 2019, rectal swabs from 266 calves across 24 farms were collected, and resistant E. coli were isolated using cefotaxime-supplemented McConkey agar. Susceptibility to 13 antibiotics was assessed by disc diffusion, and resistance genes were detected by PCR. A total of 128 (48%) E. coli isolates were obtained, with 90% (115/128) being multidrug-resistant. All isolates were resistant to ampicillin, and 127 to cephalotin. Resistance rates to other antimicrobials ranged from 13% (gentamicin) to 73% (tetracycline). ESBL production was confirmed in 91% (117/128) of isolates, while 36% (46/128) produced AmpC. PCR analysis revealed the presence of blaCTX-M (90%), blaTEM (48%), and qnrS (21%) genes. Whole-genome sequencing (WGS) was performed on 68 isolates from 16 farms. Our findings highlight the high prevalence of multidrug-resistant E. coli carrying clinically relevant resistance genes, emphasizing the need for antimicrobial stewardship in animal production.

Název v anglickém jazyce

Identification of E. coli resistant to beta-lactam antibiotics isolated from Czech diary calves

Popis výsledku anglicky

Antimicrobial resistance is a major threat to human and animal health. Our study focused on beta-lactam resistance in Escherichia coli from calves in Czech farms. In 2019, rectal swabs from 266 calves across 24 farms were collected, and resistant E. coli were isolated using cefotaxime-supplemented McConkey agar. Susceptibility to 13 antibiotics was assessed by disc diffusion, and resistance genes were detected by PCR. A total of 128 (48%) E. coli isolates were obtained, with 90% (115/128) being multidrug-resistant. All isolates were resistant to ampicillin, and 127 to cephalotin. Resistance rates to other antimicrobials ranged from 13% (gentamicin) to 73% (tetracycline). ESBL production was confirmed in 91% (117/128) of isolates, while 36% (46/128) produced AmpC. PCR analysis revealed the presence of blaCTX-M (90%), blaTEM (48%), and qnrS (21%) genes. Whole-genome sequencing (WGS) was performed on 68 isolates from 16 farms. Our findings highlight the high prevalence of multidrug-resistant E. coli carrying clinically relevant resistance genes, emphasizing the need for antimicrobial stewardship in animal production.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů