Effects of cadmium(II) ions on early somatic embryos of norway spruce studied by using electrochemical techniques and nuclear magnetic resonance

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F10%3A00002745" target="_blank" >RIV/62157124:16270/10:00002745 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081731:_____/10:00351433 RIV/62156489:43210/10:00169162 RIV/62157124:16370/10:00002645

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effects of cadmium(II) ions on early somatic embryos of norway spruce studied by using electrochemical techniques and nuclear magnetic resonance

Popis výsledku v původním jazyce

Studying of metal ions effects on plant cells and whole plants are still topical. Early somatic embryos (ESEs) of spruce represent the unique plant model system, which can be used to study of various types of environmental stresses including metal ions under well controlled experimental conditions. In the paper nuclear magnetic resonance (NMR) and electrochemical techniques were employed to investigate the effect of cadmium(II) ions (0, 50, 150, 250, 500 and 1,000 ?M) on early static embryos (ESEs) of Norway spruce, clone 2/32. The ESEs were treated with cadmium(II) ions for fourteen days. The increase in cluster area and viability were determined by image analysis and fluorescein diacetate/propidium iodide double staining, respectively. Growth characteristics revealed the ESEs treated with cadmium-ethylene diamine tetra-acetic acid (Cd-EDTA) showed an increase in growth-inhibiting effect due to cadmium(II). Furthermore, total thiol content and certain low molecular mass plant thiols (

Název v anglickém jazyce

Effects of cadmium(II) ions on early somatic embryos of norway spruce studied by using electrochemical techniques and nuclear magnetic resonance

Popis výsledku anglicky

Studying of metal ions effects on plant cells and whole plants are still topical. Early somatic embryos (ESEs) of spruce represent the unique plant model system, which can be used to study of various types of environmental stresses including metal ions under well controlled experimental conditions. In the paper nuclear magnetic resonance (NMR) and electrochemical techniques were employed to investigate the effect of cadmium(II) ions (0, 50, 150, 250, 500 and 1,000 ?M) on early static embryos (ESEs) of Norway spruce, clone 2/32. The ESEs were treated with cadmium(II) ions for fourteen days. The increase in cluster area and viability were determined by image analysis and fluorescein diacetate/propidium iodide double staining, respectively. Growth characteristics revealed the ESEs treated with cadmium-ethylene diamine tetra-acetic acid (Cd-EDTA) showed an increase in growth-inhibiting effect due to cadmium(II). Furthermore, total thiol content and certain low molecular mass plant thiols (

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International journal of electrochemical science

ISSN

1452-3981

e-ISSN

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Svazek periodika

5

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

15

Strana od-do

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Kód UT WoS článku

000283999000002

EID výsledku v databázi Scopus

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