Conflicting results of serological, PCR and microscopic methods clarify the various risk levels of canine babesiosis in Slovakia: A complex approach to Babesia canis diagnostics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F13%3A43872442" target="_blank" >RIV/62157124:16270/13:43872442 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16170/13:43872442 RIV/62157124:16810/13:43872442

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.vetpar.2012.09.016" target="_blank" >http://dx.doi.org/10.1016/j.vetpar.2012.09.016</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vetpar.2012.09.016" target="_blank" >10.1016/j.vetpar.2012.09.016</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Conflicting results of serological, PCR and microscopic methods clarify the various risk levels of canine babesiosis in Slovakia: A complex approach to Babesia canis diagnostics

Popis výsledku v původním jazyce

We have performed a survey of Babesia canis prevalence within group of dogs living in Southern and Western Slovakia. Blood samples and sera from 217 dogs, including individuals suspected of having babesiosis, were examined by nested PCR-RFLP, light microscopy and indirect fluorescence antibody test (IFAT). The detection of B. canis DNA revealed the highest number of infected dogs in the region of Nave Zamky, with 23 B. canis-Positive blood samples (35.4%, n = 65), followed by an area close to Komarno (both areas of Southern Slovakia), where 1 dog out of 52 collected (1.9%) had detectible B. canis DNA in the blood stream. The serological method revealed an opposing pattern, with only 3 dogs (4.8%, n = 63) sampled at Nove Zamky presenting IgG antibodiesagainst B. canis, while in Komarno region such antibodies were detected in 15 dogs (28.8%, n = 52). This discrepancy may be because the majority of samples from Nove Zamky were dogs suspected of an acute phase of canine babesiosis, wherea

Název v anglickém jazyce

Conflicting results of serological, PCR and microscopic methods clarify the various risk levels of canine babesiosis in Slovakia: A complex approach to Babesia canis diagnostics

Popis výsledku anglicky

We have performed a survey of Babesia canis prevalence within group of dogs living in Southern and Western Slovakia. Blood samples and sera from 217 dogs, including individuals suspected of having babesiosis, were examined by nested PCR-RFLP, light microscopy and indirect fluorescence antibody test (IFAT). The detection of B. canis DNA revealed the highest number of infected dogs in the region of Nave Zamky, with 23 B. canis-Positive blood samples (35.4%, n = 65), followed by an area close to Komarno (both areas of Southern Slovakia), where 1 dog out of 52 collected (1.9%) had detectible B. canis DNA in the blood stream. The serological method revealed an opposing pattern, with only 3 dogs (4.8%, n = 63) sampled at Nove Zamky presenting IgG antibodiesagainst B. canis, while in Komarno region such antibodies were detected in 15 dogs (28.8%, n = 52). This discrepancy may be because the majority of samples from Nove Zamky were dogs suspected of an acute phase of canine babesiosis, wherea

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Parasitology

ISSN

0304-4017

e-ISSN

—

Svazek periodika

191

Číslo periodika v rámci svazku

3-4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

353-357

Kód UT WoS článku

000314073200017

EID výsledku v databázi Scopus

—