Establishment of Myotis myotis cell lines - model for investigation of host-pathogen interaction in a natural host for emerging viruses
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F14%3A43872889" target="_blank" >RIV/62157124:16270/14:43872889 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/68081766:_____/14:00432712 RIV/00216224:14310/14:00100366
Výsledek na webu
<a href="http://dx.doi.org/10.1371/journal.pone.0109795" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0109795</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0109795" target="_blank" >10.1371/journal.pone.0109795</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Establishment of Myotis myotis cell lines - model for investigation of host-pathogen interaction in a natural host for emerging viruses
Popis výsledku v původním jazyce
Bats are found to be the natural reservoirs for many emerging viruses. In most cases, severe clinical signs caused by such virus infections are normally not seen in bats. This indicates differences in the virus-host interactions and underlines the necessity to develop natural host related models to study these phenomena. Due to the strict protection of European bat species, immortalized cell lines are the only alternative to investigate the innate anti-virus immune mechanisms. Here, we report about theestablishment and functional characterization of Myotis myotis derived cell lines from different tissues: brain (MmBr), tonsil (MmTo), peritoneal cavity (MmPca), nasal epithelium (MmNep) and nervus olfactorius (MmNol) after immortalization by SV 40 largeT antigen. The usefulness of these cell lines to study antiviral responses has been confirmed by analysis of their susceptibility to lyssavirus infection and the mRNA patterns of immune-relevant genes after poly I:C stimulation. Performe
Název v anglickém jazyce
Establishment of Myotis myotis cell lines - model for investigation of host-pathogen interaction in a natural host for emerging viruses
Popis výsledku anglicky
Bats are found to be the natural reservoirs for many emerging viruses. In most cases, severe clinical signs caused by such virus infections are normally not seen in bats. This indicates differences in the virus-host interactions and underlines the necessity to develop natural host related models to study these phenomena. Due to the strict protection of European bat species, immortalized cell lines are the only alternative to investigate the innate anti-virus immune mechanisms. Here, we report about theestablishment and functional characterization of Myotis myotis derived cell lines from different tissues: brain (MmBr), tonsil (MmTo), peritoneal cavity (MmPca), nasal epithelium (MmNep) and nervus olfactorius (MmNol) after immortalization by SV 40 largeT antigen. The usefulness of these cell lines to study antiviral responses has been confirmed by analysis of their susceptibility to lyssavirus infection and the mRNA patterns of immune-relevant genes after poly I:C stimulation. Performe
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
GJ - Choroby a škůdci zvířat, veterinární medicina
OECD FORD obor
—
Návaznosti výsledku
Projekt
—
Návaznosti
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
PLoS ONE
ISSN
1932-6203
e-ISSN
—
Svazek periodika
9
Číslo periodika v rámci svazku
10
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
10
Strana od-do
"nestrankovano"
Kód UT WoS článku
000345204000096
EID výsledku v databázi Scopus
—