Effects of the non-steroidal anti-inflammatory drug(NSAID) naproxen on gene expression of antioxidant enzymes in zebrafish (Danio rerio)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F15%3A43873962" target="_blank" >RIV/62157124:16270/15:43873962 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.etap.2015.07.009" target="_blank" >http://dx.doi.org/10.1016/j.etap.2015.07.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.etap.2015.07.009" target="_blank" >10.1016/j.etap.2015.07.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of the non-steroidal anti-inflammatory drug(NSAID) naproxen on gene expression of antioxidant enzymes in zebrafish (Danio rerio)

Popis výsledku v původním jazyce

The aim of this study was to investigate the effects of naproxen on gene expression of antioxidant enzymes of adult zebrafish. Surprisingly, after two weeks exposure no significant effect on mRNA expression of the target genes was found in liver. However, mRNA levels of three genes were altered significantly in intestine. Expression of Ucp-2 decreased at the environmental concentration of 1 ?g/L while mRNA expression of GST p2 increased at the concentration of 100 ?g/L. The mRNA level for antioxidant enzyme CAT was up-regulated significantly at both concentrations used. Exposure to naproxen caused only moderate effects on expression of antioxidant genes rather in intestine than in liver, which demonstrates that intestine is more sensitive to waterbornenaproxen exposure than liver. Interestingly, adverse side effects of NSAIDs occur in the gastrointestinal tract of humans. To our knowledge, this is the first study focused on transcriptional effects of naproxen on zebrafish.

Název v anglickém jazyce

Effects of the non-steroidal anti-inflammatory drug(NSAID) naproxen on gene expression of antioxidant enzymes in zebrafish (Danio rerio)

Popis výsledku anglicky

The aim of this study was to investigate the effects of naproxen on gene expression of antioxidant enzymes of adult zebrafish. Surprisingly, after two weeks exposure no significant effect on mRNA expression of the target genes was found in liver. However, mRNA levels of three genes were altered significantly in intestine. Expression of Ucp-2 decreased at the environmental concentration of 1 ?g/L while mRNA expression of GST p2 increased at the concentration of 100 ?g/L. The mRNA level for antioxidant enzyme CAT was up-regulated significantly at both concentrations used. Exposure to naproxen caused only moderate effects on expression of antioxidant genes rather in intestine than in liver, which demonstrates that intestine is more sensitive to waterbornenaproxen exposure than liver. Interestingly, adverse side effects of NSAIDs occur in the gastrointestinal tract of humans. To our knowledge, this is the first study focused on transcriptional effects of naproxen on zebrafish.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental toxicology and pharmacology

ISSN

1382-6689

e-ISSN

—

Svazek periodika

40

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

343-348

Kód UT WoS článku

000362619100001

EID výsledku v databázi Scopus

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