Characterisation of IncA/C-2 plasmids carrying an In416-like integron with the bla(VIM-19) gene from Klebsiella pneumoniae ST383 of Greek origin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F16%3A43874289" target="_blank" >RIV/62157124:16270/16:43874289 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/16:10316335 RIV/62157124:16810/16:43874289

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ijantimicag.2015.12.001" target="_blank" >http://dx.doi.org/10.1016/j.ijantimicag.2015.12.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijantimicag.2015.12.001" target="_blank" >10.1016/j.ijantimicag.2015.12.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterisation of IncA/C-2 plasmids carrying an In416-like integron with the bla(VIM-19) gene from Klebsiella pneumoniae ST383 of Greek origin

Popis výsledku v původním jazyce

The complete nucleotide sequences of three multidrug resistance (MDR) IncA/C-like plasmids from Enterobacteriaceae isolates carrying the VIM-type carbapenemase-encoding integrons In4863 (bla(VIM-19)-aacA7-dfrA1-Delta aadA1-smr2) or In4873 (bla(VIM-1)-aacA7-dfrA1-Delta aadA1-smr2) were determined, which are the first In416-like elements identified in Greece. Plasmids pKP-Gr642 and pKP-Gr8143 were from Klebsiella pneumoniae ST383 isolates, whereas plasmid pEcl-Gr4873 was from an Enterobacter cloacae ST88 isolate. Sequencing showed that pKP-Gr642 (162 787 bp) and pKP-Gr8143 (154 395 bp) consisted of the type 1 IncA/C-2 conserved backbone, the blaCMY-2-like gene-containing region, and the ARI-B (with the sul2 gene) and ARI-A (with a class 1 integron) resistance islands, like the plasmid pUMNK88-161 from the USA. The third plasmid, pEcl-Gr4873 (153 958 bp), exhibited extensive similarity with the type 2 IncA/C-2 plasmid pR55 from France. pEcl-Gr4873 carried only one resistance island of a hybrid transposon structure inserted in a different location to ARI-A in type 1 A/C-2 plasmids. In all three plasmids, the In416-like integrons In4863 or In4873 were identified within non-identical class II transposon structures. All three In416-like-carrying regions presented significant similarities with the MDR region of the IncA/C-2 plasmid pCC416 from Italy, carrying the prototype In416 integron (bla(VIM-4)-aacA7-dfrA1-Delta aadA1-smr2). These findings provided the basis for speculations regarding the evolution of IncA/C-2 plasmids with In416-like integrons, and confirmed the rapid evolution of some IncA/C-2 plasmid lineages. Considering the broad host range of IncA/C-2 molecules, it seems that pKP-Gr642, pKP-Gr8143 and pEcl-Gr4873 plasmids might support the diffusion of In416-like integrons among Enterobacteriaceae.

Název v anglickém jazyce

Characterisation of IncA/C-2 plasmids carrying an In416-like integron with the bla(VIM-19) gene from Klebsiella pneumoniae ST383 of Greek origin

Popis výsledku anglicky

The complete nucleotide sequences of three multidrug resistance (MDR) IncA/C-like plasmids from Enterobacteriaceae isolates carrying the VIM-type carbapenemase-encoding integrons In4863 (bla(VIM-19)-aacA7-dfrA1-Delta aadA1-smr2) or In4873 (bla(VIM-1)-aacA7-dfrA1-Delta aadA1-smr2) were determined, which are the first In416-like elements identified in Greece. Plasmids pKP-Gr642 and pKP-Gr8143 were from Klebsiella pneumoniae ST383 isolates, whereas plasmid pEcl-Gr4873 was from an Enterobacter cloacae ST88 isolate. Sequencing showed that pKP-Gr642 (162 787 bp) and pKP-Gr8143 (154 395 bp) consisted of the type 1 IncA/C-2 conserved backbone, the blaCMY-2-like gene-containing region, and the ARI-B (with the sul2 gene) and ARI-A (with a class 1 integron) resistance islands, like the plasmid pUMNK88-161 from the USA. The third plasmid, pEcl-Gr4873 (153 958 bp), exhibited extensive similarity with the type 2 IncA/C-2 plasmid pR55 from France. pEcl-Gr4873 carried only one resistance island of a hybrid transposon structure inserted in a different location to ARI-A in type 1 A/C-2 plasmids. In all three plasmids, the In416-like integrons In4863 or In4873 were identified within non-identical class II transposon structures. All three In416-like-carrying regions presented significant similarities with the MDR region of the IncA/C-2 plasmid pCC416 from Italy, carrying the prototype In416 integron (bla(VIM-4)-aacA7-dfrA1-Delta aadA1-smr2). These findings provided the basis for speculations regarding the evolution of IncA/C-2 plasmids with In416-like integrons, and confirmed the rapid evolution of some IncA/C-2 plasmid lineages. Considering the broad host range of IncA/C-2 molecules, it seems that pKP-Gr642, pKP-Gr8143 and pEcl-Gr4873 plasmids might support the diffusion of In416-like integrons among Enterobacteriaceae.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Antimicrobial Agents

ISSN

0924-8579

e-ISSN

—

Svazek periodika

47

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

158-162

Kód UT WoS článku

000369595600009

EID výsledku v databázi Scopus

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